摘要
为研究双组分信号转导系统CpxAR对鼠伤寒沙门菌生物被膜形成能力的调控作用,以鼠伤寒沙门菌的标准菌株JS和构建的cpxR缺失株JSΔcpxR、以及前期研究临床分离的6株鼠伤寒沙门菌和构建的6株cpxR基因缺失株为研究对象,首先采用PCR的方法对菌株的cpxR基因进行扩增鉴定,然后采用结晶紫染色法测定各菌株的生物被膜形成能力,最后分析cpxR基因缺失前后鼠伤寒沙门菌生物被膜形成能力的变化。结果发现,所有亲本株和cpxR基因缺失株的cpxR基因PCR扩增条带大小与预期一致。生物被膜测定结果显示,鼠伤寒沙门菌标准菌株(JS)为中等成膜能力菌株,其cpxR基因缺失株(JSΔcpxR)为弱成膜能力菌株,cpxR缺失后生物被膜形成量明显降低,差异显著(p<0.05);临床分离的鼠伤寒沙门菌中,cpxR基因缺失株SH2ΔcpxR和SH4ΔcpxR与其亲本株(SH2和SH4)相比,生物被膜形成量明显降低,差异显著(p<0.05);cpxR基因缺失株(SH3ΔcpxR和SH7ΔcpxR)与其亲本株SH3和SH7相比,生物被膜形成量显著降低,差异极显著(p<0.01)。结果表明,cpxR基因缺失可使鼠伤寒沙门菌的成膜能力明显降低,该基因在鼠伤寒沙门菌生物被膜形成过程中发挥着重要作用。
In order to explore the role of the CpxAR system,a two-component signal transduction system,in biofilm formation of Salmonella typhimurium,the standard strain JS of Salmonella typhimurium and the cpxR deletion strain JSΔcpxR constructed in the previous study,6 Salmonella typhimurium strains detached in the previous study and 6 cpxR gene deletion strains were selected as the research objects.Firstly,the cpxR gene was amplified and identified by PCR,and then the biofilm formation energy of each strain was determined by crystal violet staining.Finally,the changes of biofilm formation ability of Salmonella typhimurium before and after cpxR gene deletion were analyzed.The results showed that the PCR amplification bands of cpxR gene of all parents and cpxR gene deletion strains were consistent with the expected size.The biofilm analysis showed that the standard strain(JS)was a medium film-forming strain,while its cpxR gene deletion strain JSΔcpxR was a weak film-forming ability strain,and the amount of biofilm formation decreased significantly after cpxR deletion(p<0.05).In clinical isolates of Salmonella typhimurium,the amount of the biofilm formation of cpxR deleted strains SH2ΔcpxR and SH4ΔcpxR was significantly lower than that of their parents SH2 and SH4(p<0.05),and the biofilm formation of cpxR deleted strains SH3ΔcpxR and SH7ΔcpxR was extremely significantly lower than that of their parents SH3 and SH7(p<0.01).The results indicated that the deletion of cpxR gene could significantly reduce the biofilm formation ability of Salmonella typhimurium,therefore cpxR gene played an important role in the biofilm formation of Salmonella typhimurium.
作者
黄慧
樊国燕
彭志锋
娄飞
王晓琳
裴亚玲
胡功政
HUANG Hui;FAN Guoyan;PENG Zhifeng;LOU Fei;WANG Xiaolin;PEI Yaling;HU Gongzheng(College of Animal Medicine,Henan University of Animal Husbandry and Economy,Zhengzhou Henan 450046;College of Animal Medicine,Henan Agricultural University,Zhengzhou Henan 450046)
出处
《现代牧业》
2021年第1期17-21,共5页
Modern Animal Husbandry
基金
河南牧业经济学院博士科研启动基金项目(2018HNUAHEDF016)
河南省科技攻关计划项目(182102110229)。
