摘要
为了解海南省海口市美兰区市售生鲜肉沙门氏菌污染情况,笔者采集了海口市美兰区市售猪肉、鸡肉和鸭肉样本共50份,进行了沙门氏菌分离、Kindy-Bauer药敏试验及耐药基因分析。结果显示:共得到4份沙门氏菌菌株,检出率为8%。4株沙门氏菌对15种抗生素产生不同耐药性,其中,对青霉素、氨苄西林、四环素和吉他霉素耐药率为100%,对氯霉素、阿莫西林和磺胺异噁唑耐药率为75%,对多西环素、红霉素、卡那霉素、诺氟沙星和链霉素耐药率达到50%。PCR结果显示,4株沙门氏菌菌株对四环素类耐药基因tetA检出率为50%,对磺胺类药物耐药基因sul2和喹诺酮类耐药基因qurB基因检出率为0。本研究结果表明,海口局部地区禽畜类生鲜肉类沙门氏菌感染率较低,但分类菌株具有较广耐药性,应加强监管。
In order to reveal the pollution situation of salmonella in fresh livestock and poultry meats in Meilan district of Haikou City,Hainan Province a total of 50 samples of pork,chicken and duck meats from inside and outside of the market were collected for salmonella isolation,Kindy-Bauer antibiotic sensitivity test and antibiotic resistance gene analysis.The results showed that 4 strains of salmonella were isolated from the 50 samples with a detection rate of 8%.The 4salmonella isolates exhibited different levels of sensitivity to 15 antibiotics:100%resistant to penicillin,ampicillin,tetracycline and kitasamycin,75%resistant to chloramphenicol,amoxicillin and sulfisoxazole and upto 50%resistant to doxycycline,erythromycin,kanamycin,norfloxacin and streptomycin but not sensitive to ciprofloxacin.Meanwhile,the detection rate of the 4 salmonella isolates for sulfonamide resistance gene sul2 and quinolone resistance gene qurB gene was 0,and the detection rate for tetracycline resistance tetA gene was 50%.It is concluded that the fresh meats of livestock and poultry in Haikou infected with salmonella was not popular,but these isolates had a wide resistance to multi-antibiotics.It is necessary to strengthen the supervision of food safety and reduce the abuse of antibiotics.
作者
郭定乾
罗瑛
洪枫
满初日嘎
GUO Dingqian;LUO Ying;HONG Feng;MANCHU Riga(Hainan University,College of Animal Science and Technology,Haikou,Hainan 570228,China)
出处
《热带生物学报》
2021年第1期103-109,共7页
Journal of Tropical Biology
基金
海南大学资助项目(hdjy1929,hdjy2062)。
关键词
沙门氏菌
生鲜肉
耐药性
海口
Salmonella
fresh meat
antibiotic resistance
