摘要
为明确马铃薯新品系染色体构型及在DNA水平上的遗传差异。以5个马铃薯新品系(NNS-01、NNS-02、NNS-03、NNS-04、NNS-05)及对照(MIN-021)为材料,采用常规压片法及SSR分子标记技术对其PMCMⅠ染色体配对构型及SSR多态性进行了分析。结果表明,5个马铃薯新品系的染色体构型明显不同,依次为7.66Ⅰ+7.52Ⅱ+6.82Ⅲ+1.21Ⅳ、4.72Ⅰ+11.02Ⅱ+3.2Ⅲ+2.91Ⅳ、5.26Ⅰ+10.2Ⅱ+3.82Ⅲ+2.72Ⅳ、8.39Ⅰ+7.32Ⅱ+6.51Ⅲ+1.36Ⅳ和4.96Ⅰ+8.06Ⅱ+5.74Ⅲ+2.43Ⅳ。利用筛选出的10对SSR适宜引物,对各供试材料的基因组DNA进行PCR扩增,获得SSR多态性条带94个,多态性比率为72.23%;用特异性引物S 189建立了能明显鉴别5个新品系及对照材料的SSR指纹图;以GD值0.39为基准,将6个材料分成四类:新品系NNS-01和NNS-02为一类,新品系NNS-03和对照材料MIN-021为一类,NNS-04和NNS-05各单独为一类。
In order to clarify the difference of chromosome configuration and its genetic differences at DNA level of potato lines,the five new potato lines(NNS-01,NNS-02,NNS-03,NNS-04 and NNS-05)and control(MIN-021)were selected as materials to study PMCMⅠchromosome paired configuration and SSR polymorphisms by conventional tablet pressing method and SSR molecular marker technology.The results showed that the chromosome configuration of the five new potato lines were obviously different,followed by 7.66Ⅰ+7.52Ⅱ+6.82Ⅲ+1.21Ⅳ,4.72Ⅰ+11.02Ⅱ+3.2Ⅲ+2.91Ⅳ,5.26Ⅰ+10.2Ⅱ+3.82Ⅲ+2.72Ⅳ,8.39Ⅰ+7.32Ⅱ+6.51Ⅲ+1.36Ⅳand 4.96Ⅰ+8.06Ⅱ+5.74Ⅲ+2.43Ⅳ.Meanwhile,10 pairs of SSR primers were selected to amplify the genomic DNA of each tested material by PCR,and 94 SSR polymorphism bands were obtained,with a polymorphism ratio of 72.23%.SSR fingerprint were established with specific primers S 189 for the identification of 5 new lines and control materials.Based on the GD value of 0.39,the six materials were divided into four categories.And,new lines NNS-01 and NNS-02 were classified as one category and new line NNS-03 and control material MIN-021 were classified as one category,and NNS-04 and NNS-05 were respectively classified as one category.
作者
刘宇飞
于卓
于肖夏
杨东升
李佳奇
李景伟
卢倩倩
李靓
LIU Yufei;YU Zhuo;YU Xiaoxia;YANG Dongsheng;LI Jiaqi;LI Jingwei;LU Qianqian;LI Liang(Agronomy College,Inner Mongolia Agricultural University,Huhhot 010019,China)
出处
《种子》
北大核心
2021年第2期53-57,共5页
Seed
基金
内蒙古自治区科技重大专项(ZDZX 2018019)
内蒙古农业大学科技成果转化专项资金动植物品种培育项目(YZGC 2017006)
内蒙古自治区自然科学基金面上项目(2018 MS 03040)
内蒙古自治区高等学校青年科技英才支持计划(NJYT-18-B 04)资助
