摘要
目的探讨长链非编码RNA(long noncoding RNA,lncRNA)前列腺相关转录本6(prostate cancer associated transcript 6,PCAT6)在胰腺癌组织和细胞中的表达情况及其调控胰腺癌细胞增殖、侵袭和迁移的分子机制。方法采用qRT-PCR法检测胰腺癌组织、癌旁正常组织和胰腺癌细胞系中PCAT6和miR-185-5p的表达水平和两者相对关系。采用CCK-8、Transwell实验检测胰腺癌细胞增殖、转移和侵袭能力。生物信息学预测和双荧光素酶报告基因实验证实PCAT6与miR-185-5p间的靶向关系。结果PCAT6在胰腺癌肿瘤组织中表达量高于癌旁正常组织(P<0.05),高表达的PCAT6与胰腺癌TNM分期(P<0.05)和淋巴结转移(P<0.05)密切相关。外源性沉默PCAT6可以抑制胰腺癌细胞的增殖、侵袭和转移能力。PCAT6-WT与miR-185-5p mimics共转染后荧光素酶活性显著降低(P<0.05),而PCAT6-WUT与miR-185-5p mimics共转染组的荧光素酶活性无明显变化。结论LncRNA PCAT6通过靶向miR-185-5p调控胰腺癌的增殖、迁移和侵袭。
Objective To investigate the expression of long noncoding RNA(lncRNA)prostate cancer associated transcript 6(PCAT6)in pancreatic carcinoma tissues and cells and its molecular mechanism of regulating proliferation,migration and invasion in pancreatic carcinoma cells.Methods The PCAT6 and miR-185-5p expression in pancreatic carcinoma tissues,paired adjacent normal tissues and pancreatic carcinoma cells was detected by qRT-PCR.CCK8,Transwell assay was used to detect the ability proliferation,migration and invasion of pancreatic carcinoma cells.Bioinformatics prediction and dual luciferase reporter gene assay were used to demonstrate the targeting relationship between PCAT6 and miR-185-5p.Results PCAT6 was up-regulated in pancreatic carcinoma compared with adjacent normal tissue and over-expression of PCAT6 was correlated with advanced TNM stage(P<0.05)and lymph node invasion(P<0.05).The exogenous silencing of PCAT6 could inhibit the proliferation,migration and invasion of pancreatic carcinoma cells.The activity of luciferase significantly reduced after co-transfection of PCAT6-WT and miR-185-5p mimics(P<0.05),but there was no obviously change of luciferase activity when PCAT6-WUT and miR-185-5p mimics co-transfected.Conclusion LncRNA PCAT6 regulates proliferation,migration and invasion of pancreatic carcinoma cells by targeting miR-185-5p.
作者
赵俞乔
刘浪
王健岗
关沧海
王东升
钟翔宇
姜兴明
ZHAO Yuqiao;LIU Lang;WANG Jian′gang;GUAN Canghai;WANG Dongsheng;ZHONG Xiangyu;JIANG Xingming(Department of General Surgery,the Second Affiliated Hospital of Harbin Medical University,Harbin 150086,China)
出处
《实用医学杂志》
CAS
北大核心
2021年第5期620-625,共6页
The Journal of Practical Medicine
基金
黑龙江省自然科学基金(编号:LH2020H058)
湖北陈孝平科技发展基金(编号:CXPJJH12000002⁃2020015)
国家自然科学基金(编号:81602088)。
