摘要
目的:基于大鼠离体外翻肠囊模型探讨豆腐果苷在不同肠段的吸收情况。方法:建立豆腐果苷及其代谢产物高效液相色谱同时检测方法。在不同肠段(十二指肠、空肠、回肠、结肠)的外翻肠囊模型中加入含豆腐果苷的Krebs-Ringer液,不同时间点(5,10,15,30,45,60,75,90 min)取样并测定囊内药物浓度,比较四个肠段对豆腐果苷的吸收情况。结果:所建立的高效液相色谱法成功应用于豆腐果苷及其代谢产物同时检测。豆腐果苷及其代谢产物吸收迅速并呈明显的时间依赖性,十二指肠段的吸收与代谢情况均高于其他肠段。结论:十二指肠肠段是豆腐果苷分肠段吸收与代谢的主要部位,这是豆腐果苷肠段代谢研究的首次报道。
AIM:To investigate the absorption of helicid in different segments of intestine based on rat everted intestine sac model.METHODS:To establish a high-performance liquid chromatography method for simultaneous determination of helicid and its metabolite.Krebs-ringer solution containing helicid was added to everted intestine sacs of different segments(duodenum,Jejunum,ileum and colon).Drug concentration in sacs was determined at different time points(5,10,15,30,45,60,75,90 min).Adsorptions of helicid in four intestinal segments were compared.RESULTS:This high-performance liquid chromatography was successfully applied to the simultaneous determination of helicid and its metabolite.Absorption of helicid was rapid and time-dependent.The absorption and metabolism of helicid in duodenum segment were higher than these in other segments.CONCLUSION:The duodenum segment is the main site of segmental absorption and metabolism of helicid.This is the first report on intestinal segment metabolism of helicid.
作者
王萍
沈杰
储冀汝
郭南
谢海棠
詹翠娇
王昌茂
杨斌
郑丹丹
胡容峰
贾元威
WANG Ping;SHEN Jie;CHU Jiru;GUO Nan;XIE Haitang;ZHAN Cuijiao;WANG Changmao;YANG Bin;ZHENG Dandan;HU Rongfeng;JIA Yuanwei(Pharmacy College,Wannan Medical College,Wuhu 241001,Anhui,China;Drug Clinical Evaluation Center of Anhui Province,Yijishan Hospital of Wannan Medical College,Wuhu 241001,Anhui,China;Department of Pharmacy,Yijishan Hospital of Wannan Medical College,Wuhu 241001,Anhui,China;Key Laboratory of Xin'an Medicine,Ministry of Education,Hefei 230000,Anhui,China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2021年第3期258-263,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
国家自然科学基金项目(81973488
81503236)
弋矶山医院引进人才科研基金资助项目(YR201913
YR202015)
皖南医学院弋矶山医院科研能力“高峰”培育计划(GF2019J05
GF2019J06)
皖南医学院弋矶山医院科技创新团队“攀峰”培育计划(KPF2019016)
皖南医学院校中青年基金项目(WK2018F04
WK2019F35)。
关键词
豆腐果苷
肠囊外翻模型
吸收
代谢
helicid
everted intestinal sac model
absorption
metabolism
