摘要
目的基于多元活性成分同时测定结合多元统计分析综合评价不同产地连翘药材的质量。方法采用超快速液相色谱-三重四极杆/线性离子阱质谱法(UFLC-QTRAP-MS/MS)同时测定不同产地连翘药材中苯乙醇苷、木脂素、黄酮及酚酸类共21种活性成分的含量;根据21种目标成分的含量,用聚类分析(HCA)、主成分分析(PCA)及单因素方差分析(One-way ANOVA)对不同产地连翘药材进行综合评价。结果 HCA结果显示,连翘3个主产区14批药材样品分为2类,第1类为产地山西陵川、陕西合阳、河南辉县、河南林川和河南洛阳,第2类为产地山西安泽和山西古县;PCA结果显示,山西产连翘药材的综合质量优于陕西和河南;One-way ANOVA结果显示,所测成分中,绿原酸、阿魏酸、紫云英苷、牛蒡子苷、木犀草素、连翘酯苷B、连翘酯苷A、松脂醇-β-D-葡萄糖苷、连翘苷和松脂醇10种成分在连翘3个主产区样品间存在显著差异。结论所构建的多元活性成分同时测定结合多元统计分析的综合评价体系,成功地应用于不同产地连翘药材的质量评价,可为连翘药材内在质量的综合评价和全面控制提供新的方法参考,亦为连翘药材的道地性研究提供基础资料。
OBJECTIVE To comprehensively evaluate the quality of Forsythiae Fructus from different habitats based on simultaneous determination of multiple active components combined with multivariate statistical analysis. METHODS Twenty-one bioactive constituents including phenylethanoid glycosides, lignans, flavonoids and phenolic acids in Forsythiae Fructus from different producing areas were simultaneously determined by ultra-fast liquid chromatography coupled with triple quadrupole/linear ion trap tandem mass spectrometry(UFLC-QTRAP-MS/MS). Based on the contents of 21 target components, hierarchical cluster analysis(HCA), principal component analysis(PCA) and One-way analysis of variance(One-way ANOVA) were further employed for the quality assessment of Forsythiae Fructus from different habitats. RESULTS The HCA results showed that 14 batches of medicinal materials from three major producing areas were divided into two categories: the first category was Lingchuan in Shanxi, Heyang in Shaanxi, Huixian in Henan, Linchuan in Henan and Luoyang in Henan;the second category was Anze in Shanxi and Guxian in Shanxi. The PCA results revealed that the comprehensive quality of Forsythiae Fructus from Shanxi was better than those from Shaanxi and Henan. Additionally, the results of One-way ANOVA indicated that the contents of 10 components, ie chlorogenic acid, ferulic acid, astragalin, arctiin, luteolin, forsythiaside B, forsythiaside A, pinoresinol-4-O-β-D-glucoside, phillyrin and pinoresinol were significantly different among the above three provinces. CONCLUSION The integrated evaluation system based on simultaneous determination of multiple bioactive constituents combined with multivariate statistical analysis is successfully applied to the quality evaluation of Forsythiae Fructus from different habitats. It can provide a new reference method for the quality evaluation and control of Forsythiae Fructus, which can also provide basic information for further expounding the geo-authentic of Forsythiae Fructus.
作者
魏丽芳
梅余琪
邹立思
陈佳丽
谈梦霞
王程成
蔡芷辰
林丽群
刘训红
WEI Li-fang;MEI Yu-qi;ZOU Li-si;CHEN Jia-li;TAN Meng-xia;WANG Cheng-cheng;CAI Zhi-chen;LIN Li-qun;LIU Xun-hong(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China)
出处
《中国药学杂志》
CAS
CSCD
北大核心
2021年第4期276-284,共9页
Chinese Pharmaceutical Journal
基金
江苏高校优势学科建设工程项目资助(YSXK-2014)
江苏高校品牌专业建设工程项目资助(PPZY2015A070)。
