摘要
介绍了染色体分选技术的基本原理和样品处理的基本流程,对根尖分生区采用同步化处理,制备染色体悬浮液,最后通过流式细胞仪的分析与收集获得纯度高、数量多的目标染色体。综述了染色体分选技术在植物学研究中的主要应用,包括物理图谱的构建、DNA分子标记的开发、以及复杂多倍体植物的基因组测序等。通过染色体分选技术的不断完善与发展,应用于染色体分选的探针标记不断开发,以及分选后的染色体DNA纯化和扩增技术的优化,将为多倍体植物如甘蔗等基因组学研究提供更有效的帮助。
The basic principle of chromosome sorting technology and the basic process of sample treatment were introduced.The chromosome suspension was prepared by synchronizing the apical meristem.And then the target chromosomes with high purity and high quantity were obtained by flow cytometry.The main applications of chromosome sorting technology in plants were reviewed,including the construction of physical maps,the development of DNA molecular markers,and genome sequence of the complex polyploid plant.With the continuous improvement and development of chromosome sorting technology,the development of probe markers for chromosome sorting and the optimization of chromosome DNA purification and amplification technology after sorted,it will provide more effective help for the genomics research of polyploid plants such as sugarcane.
作者
钱旺
王秋松
杨善
徐良年
邓祖湖
QIAN Wang;WANG Qiu-song;YANG Shan;XU Liang-nian;DENG Zu-hu(National Engineering Research Center of Sugarcane,Fujian Agriculture and Forestry University,Fuzhou 350002,China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources,Nanning 530004,China;Guangxi Key Laboratory for Sugarcane Biology,Nanning 530004,China;Key Lab of Sugarcane Biology and Genetic Breeding,Ministry of Agriculture and Rural Affairs,Fuzhou 350002,China)
出处
《热带亚热带植物学报》
CAS
CSCD
北大核心
2021年第2期221-228,共8页
Journal of Tropical and Subtropical Botany
基金
国家自然科学基金项目(31771863)
中国现代农业技术专项基金项目(CARS-170106)
亚热带农业生物资源保护与利用国家重点实验室项目(SKLCUSA-a201912,SKLCUSA-b201806)
福建农林大学科技创新专项基金(KFA17168A,KFA17525A,KFA17169A,2018N1002)资助。
