摘要
为了探究近交对繁殖效应的影响,本研究以国家级地方鸡种基因库保存的狼山鸡为素材,结合分子标记和系谱信息,组建高、低近交两个试验组,记录高、低近交组的繁殖性能数据。选取高、低近交组中正常个体各3只,采取卵巢组织进行RNA-seq测序,并对差异基因进行功能注释。结果在高、低近交组中共获得差异转录本1114个,其中783个基因获得注释,307个上调,476个下调。GO和KEGG分析表明,差异基因主要富集在核糖体的生物合成、炎性反应、繁殖、生长、免疫系统过程、代谢过程等生物学过程,Pathway显著富集在叶酸的生物合成、卵母细胞成熟和代谢等生物学通路。功能分析发现,筛选出的差异基因(如GGH、CPEB1、GNMT和PIWIL等)与繁殖功能相关。此外,还包括一些与应激和免疫相关的基因(如APOC3、HSP70、CD38和LGMN等)。研究结果有助于了解狼山鸡繁殖性状近交衰退相关的基因及其调控机制,为家禽特定性状近交衰退分子机理提供参考。
In order to explore the effect of inbreeding on reproductive effects,this study selected Langshan chickens conserved in the National Chicken Genetic Resources as materials.Combining molecular markers and pedigre information,two groups of highly and low inbred chickens were established,and the reproductive performance data were recorded for both groups.Three chickens was selected as a material in each group,and ovarian tissues were taken for RNA-seq sequencing,and the functional annotation of different genes was carried out.The results showed that a total of 1114 differential transcripts were obtained in the highly and low inbred groups,of which 783 genes were annotated,307 were up-regulated and 476 were down-regulated.GO and KEGG analysis showed that the differential genes were mainly enriched in biological processes such as ribosome biosynthesis,inflammatory response,reproduction,growth,immune system processes,and metabolic processes.Biological pathways including in folic acid biosynthesis,oocytes maturation and metabolism were also enriched by the differential genes.Functional analysis found that the differential genes(such as GGH,CPEB1,GNMT and PIWIL)were related to reproductive function.In addition,some genes related to immunity and stress(such as APOC3,HSP70,CD38 and LGMN)were also included.The results of this study were helpful for us to understand the genes and their regulatory mechanisms related to inbreeding depression of reproductive traits in Langshan chickens,and would provide a reference for the molecular mechanism of inbreeding depression of specific traits in poultry.
作者
李国辉
曹愉夏
薛倩
张会永
殷建玫
朱云芬
沈海玉
窦新红
苏一军
王克华
邹剑敏
韩威
LI Guohui;CAO Yuxia;XUE Qian;ZHANG Huiyong;YIN Jianmei;ZHU Yunfen;SHEN Haiyu;DOU Xinhong;SU Yijun;WANG Kehua;ZOU Jianmin;HAN Wei(National Chickens Genetic Resources,Jiangsu Institute of Poultry Science,Yangzhou225125,China;The Projects of Key Laboratory for Poultry Genetics and Breeding of Jiangsu Province,Yangzhou 225125,China)
出处
《中国畜牧兽医》
CAS
北大核心
2021年第3期954-962,共9页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金面上项目(31572358)
江苏省“六大人才高峰”高层次人才项目(NY-024)
江苏省重点研发计划(现代农业)专项(BE2019353)
江苏省公益类科研院所自主科研经费项目(BM2018026)
现代农业产业技术体系建设专项资金(CARS-42-G03)
扬州市自然科学基金项目(YZ2018098)
江苏省家禽遗传育种重点实验室资助项目(JQLAB-ZZ-202007)。
关键词
狼山鸡
近交
卵巢
RNA-seq测序
差异基因
Langshan chickens
inbreeding
ovary
RNA-seq sequencing
differentially genes
