摘要
2018-2019年,在新疆喀什地区调查时发现部分种植的辣椒植株上心叶发黄皱缩、卷曲,个别植株叶片呈现斑驳花叶状。为了明确引起新疆喀什辣椒病毒病的病毒种类,提取典型症状样品的总RNA,反转录得到cDNA,分别用辣椒轻斑驳病毒和黄瓜花叶病毒特异性引物进行RT-PCR检测,CMV和PMMoV特异引物分别扩增得到约735,472 bp的特异条带,阴性对照未扩增目的条带,核苷酸序列比对发现,分别与韩国西葫芦CMV分离物(GU327368.1)和日本辣椒PMMoV分离物(AB276030.1)序列同源性最高为95%,99%,结果表明,喀什地区辣椒疑似病毒病样本被CMV与PMMoV复合侵染。
During 2018 to 2019,pepper plants showed typical symptoms of dwarf,yellowing,curling,chlorosis and rolling up of leaves were found in Kashgar,Xinjiang Uygur Autonomous region.To identify the virus that causing pepper virus disease,total RNA was isolated from the diseased tissues with typical virus symptoms,and reverse transcription(RT)-PCR assays were performed with specific primers for Cucumber mosaic virus(CMV)and Pepper mild mottle virus(PMMoV).The expected 735 bp CMV and 472 bp PMMoV fragments were amplified from diseased pepper tissue samples,respectively.Sequence analysis showed 95%,99%similarity with that of CMV(South Korea,GU327368.1)and PMMoV(Japan,AB276030.1),respectively.The results showed that pepper plants were co-infected by CMV and PMMoV in Kashgar,Xinjiang region.
作者
柴阿丽
陈利达
许帅
帕提古丽·艾斯木托拉
王利丽
石延霞
谢学文
李磊
李宝聚
CHAI Ali;CHEN Lida;XU Shuai;PATIGULI Aisimutuola;WANG Lili;SHI Yanxia;XIE Xuewen;LI Lei;LI Baoju(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;Institute of Horticulture,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China)
出处
《华北农学报》
CSCD
北大核心
2020年第S01期332-337,共6页
Acta Agriculturae Boreali-Sinica
基金
“十三五”国家重点研发计划(2016YFD0201000)
自治区区域协同创新专项(2018E02027)
大宗蔬菜产业技术体系(CARS-23)
中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)
农业部园艺作物生物学与种质创制重点实验室项目。
