摘要
叶片叶绿素和根系丙二醛含量是评价甜瓜耐低温弱光特性的重要生理指标。为了正确处理生理指标的数据,筛选甜瓜耐低温弱光极端材料,对8份甜瓜核心亲本进行低温弱光特性评价。首先通过比较2个生理指标与伤害指数(表型指标)的鉴定结果,评价2个生理指标及数据处理方式的正确性。结果显示,通过直接比较胁迫后甜瓜叶片叶绿素和根系丙二醛含量,即可正确鉴定植株耐低温弱光特性。不耐低温弱光甜瓜自交系与耐低温弱光自交系相比,叶绿素含量更低,丙二醛含量更高。通过2个指标处理前后的变幅,能够辅助了解材料对胁迫的敏感程度。其次,通过筛选,获得耐低温弱光甜瓜材料P143和敏感材料P149,用以甜瓜耐低温弱光分子标记筛选群体构建。研究结果为甜瓜耐低温弱光生理指标选择和鉴定数据处理方法的标准化提供依据,也为其他逆境胁迫数据处理起到借鉴作用。
The chlorophyll content of leave and the malonaldehyde(MDA)content of root were the important physiological index for identification of low temperature and low light in melon.In order to select the extreme material by the correct physiological data processing,eight core parental inbred lines were used to be evaluated.The evaluation results derived from two physiological index and the injury index were compared first.The injury index was calculated according to the injury classification.Two data processing methods were used in physiological data.One was using the measure value of SPAD and MDA after stress directly,another was using the absolute value of SPAD and MDA calculated between before and after stress.The compare results showed that under low temperature and low light stress,the leaf chlorosis and macerating root was happened in melon seedling.The SPAD value of poor tolerance melon inbred lines was getting smaller and the MDA content was getting larger.Using the measure value of SPAD and MDA after stress directly could get the same low temperature and low light stress tolerance results with which identified from injury index.The absolute value of SPAD and MDA calculated between before and after stress could used to evaluate accessorily the sensitive ability to stress for inbred lines.The tolerance inbred line of P143 and the sensitive line of P149 were selected for further research of molecular selection.The results were useful to uniform the low temperature and low light identification in melon and to provide reference for other stress data processing.
作者
宋慧
黄芸萍
古斌权
付玉婧
臧全宇
邢乃林
张香琴
SONG Hui;HUANG Yunping;GU Binquan;FU Yujing;ZANG Quanyu;XING Nailin;ZHANG Xiangqin(Ningbo Academy of Agricultural Sciences,Key Laboratory of Ningbo City Breeding of Cucurbitaceous Vegetables,Ningbo 315040,China;College of Agricultural and Food Science,Zhejiang Agriculture and Forestry University,Linan 311300,China)
出处
《华北农学报》
CSCD
北大核心
2020年第S01期183-188,共6页
Acta Agriculturae Boreali-Sinica
基金
现代种业科技创新专项(2019B10007)
国家自然科学基金项目(31572142,31000907)。
关键词
甜瓜
低温弱光
胁迫
Melon
Low temperature and low light
Stress
