摘要
以富油蛋白核小球藻为出发藻株,研究自养、异养和混养培养模式对小球藻生物量和油脂含量的影响,以及异养发酵培养基葡萄糖质量浓度、氮源种类及质量浓度对小球藻生长的影响。结果表明,与自养和混养培养模式相比,采用异养发酵方式培养蛋白核小球藻可获得最大的生物量和油脂含量。通过气相色谱法测得异养蛋白核小球藻油主要脂肪酸为棕榈酸(36.07%)、油酸(34.26%)、亚油酸(20.17%)和亚麻酸(6.12%)。经单因素试验优化得到最适蛋白核小球藻生长异养发酵培养基的葡萄糖质量浓度为60 g/L,最适的氮源为酵母粉,质量浓度为4 g/L,在此条件下经192 h发酵,蛋白核小球藻生物量可达12.43 g/L,油脂产量为5.45 g/L。研究结果表明,异养发酵培养获得的蛋白核小球藻油是一种潜在且可再生的新油源。
With oleaginous Chlorella pyrenoidosa as origional strain,the effects of different cultivation modes( autotrophic,heterotrophic and mixotrophic cultivations) on the biomass and oil content of the microalga and the effects of glucose mass concentration,type and mass concentration of nitrogen source in the heterotrophic medium on the growth of the microalga were investigated. The results showed that Chlorella pyrenoidosa had the highest biomass and oil content by heterotrophic cultivation in comparison to the autotrophic and mixotrophic cultivations. The main fatty acids of Chlorella pyrenoidosa oil by heterotrophic cultivation were palmitic acid( 36. 07%),oleic acid( 34. 26%),linoleic acid( 20. 17%)and linolenic acid( 6. 12%) based on the GC results. The optimal heterotrophic cultivation conditions for Chlorella pyrenoidosa growth were obtained by single factor experiment as follows: glucose mass concentration 60 g/L,with yeast as nitrogen source and the mass concentration 4 g/L. Under the optimal conditions,Chlorella pyrenoidosa achieved 12. 43 g/L of biomass and the oil yield was 5. 45 g/L after fermentation for 192 h. These results showed that Chlorella pyrenoidosa oil obtained by heterotrophic cultivation was a potential and new edible oil source.
作者
肖雪花
占凌云
戴静璇
何勇锦
陈必链
XIAO Xuehua;ZHAN Lingyun;DAI Jingxuan;HE Yongjin;CHEN Bilian(College of Life Sciences,Fujian Normal University,Fuzhou 350117,China;Engineering Research Center of Industrial Microbiology,Ministry of Education,Fujian Normal University,Fuzhou 350117,China)
出处
《中国油脂》
CAS
CSCD
北大核心
2021年第1期132-136,共5页
China Oils and Fats
基金
“十三五”海洋经济创新发展示范项目(FZHJ04)。
关键词
蛋白核小球藻
异养发酵
生物量
油脂
Chlorella pyrenoidosa
heterotrophic fermentation
biomass
oil
