摘要
为探究黄羽肉鸡鸡群临床异常的发病原因,通过病理剖检、组织病理学观察、PCR检测、细胞培养、间接免疫荧光试验(Immunofluorescent assay,IFA)、序列测定进行病原分离鉴定。结果显示:病鸡心脏、肝脏等器官均有肿瘤样病变;常见肿瘤病病毒PCR检测呈现马立克氏病病毒(Marek′s disease virus,MDV)阳性,细胞培养病毒分离及IFA鉴定结果表明成功分离一株MDV野毒株(命名为GX18NNM4);分离株meq基因的序列分析表明,GX18NNM4与vvMDV参考株GX0101的同源性最高,其核苷酸及氨基酸相似性分别高达99.8%和99.4%,且其突变位点符合国内强毒株的特征。通过氨基酸序列分析发现GX18NNM4的两个脯氨酸重复序列PPPP发生了突变,即PPPP→PNPP,且GX18NNM4与vvMDV/vv+MDV参考株RB1B、Md5、GX0101及648A的脯氨酸发生中断的数量同在0~3的范围内。结果表明该鸡群为MDV感染。
In order to explore the cause of the clinical disease in yellow-feather broilers, a series of laboratory tests were conducted to confirm the causes of the disease, including pathological anatomy, histopathological observation,PCR detection, cell cultures, immunofluorescent assay(IFA) and sequencing. The results showed that the heart, liver and other organs of the diseased chickens showed tumor-like gross lesions;Routine PCR detection of the common oncogenic viruses was the positive for Marek’s disease virus(MDV). The results of virus isolation and IFA showed that a wild strain GX18 NNM4 of MDV had been isolated. Sequence analysis based on the meq gene of MDV showed that GX18 NNM4 had the highest homology to the vvMDV strain GX0101, the nucleotide and amino acid of similarities were 99.8% and 99.4%, respectively. Amino acid alignment showed that GX18 NNM4 had two point mutations within the proline-rich repeats that interrupted stretches of four prolines at position two(PPPP→PNPP) and the interruption number of proline-repeat among GXNN18 M4 and the reference strains RB1B, Md5, GX0101 and 648 A were in the same range of 0 to 3. The results indicated that the flock was infected with MDV.
作者
李敏
高延利
石梦雅
王威威
李海娟
李秋红
邓乔木
黄腾
韦平
LI Min;GAO Yanli;SHI Mengya;WANG;Weiwei;LI Haijuan;LI Qiuhong;DENG Qiaomu;HUANG Teng;WEI Ping(Institute for Poultry Science and Health,Guangxi University,Nanning,Guangxi 530004)
出处
《中国家禽》
北大核心
2021年第3期28-34,共7页
China Poultry
基金
2017广西科技重大专项(桂科AA17204057)
国家现代农业产业技术体系广西肉鸡产业创新团队建设专项(nycytxgxcxtd-19-03)
