摘要
目的:观察井穴放血疗法对脑淋巴引流阻断缺血大鼠脑水肿的影响。方法:将雄性SD大鼠30只随机分为正常组、假手术组、模型组、井穴放血组、缺血+淋巴引流阻滞组和缺血+淋巴引流阻滞+井穴放血组,分别用Longa法和Casley-Smith法制作永久性大脑中动脉栓塞模型和脑淋巴引流阻断模型,测定6 h后各组大鼠脑含水量、脑系数和脑密度。结果:右脑含水量模型组高于井穴放血组,差异有统计学意义(P<0.05),井穴放血组右脑含水量小于缺血+淋巴引流阻滞组与缺血+淋巴引流阻滞+井穴放血组(P<0.05),缺血+淋巴引流阻滞组和缺血+淋巴引流阻滞+井穴放血组比较差异无统计学意义(P>0.05)。左脑系数各组间比较差异无统计学意义(P>0.05);除正常组和假手术组外,其余各组右脑系数与模型组比较差异均有统计学意义(P<0.05);全脑系数井穴放血组与缺血+淋巴引流阻滞组和缺血+淋巴引流阻滞+井穴放血组比较差异均有统计学意义(P<0.05),而缺血+淋巴引流阻滞组和缺血+淋巴引流阻滞+井穴放血组比较差异无统计学意义(P<0.05)。右侧脑密度模型组、缺血+淋巴引流阻滞组缺血+淋巴引流阻滞+井穴放血组与正常组比较差异有统计学意义(P<0.01)。结论:井穴放血疗法对脑缺血急性期脑水肿有一定改善作用,实施脑淋巴引流阻断后井穴放血效应减弱。
Objective:To observe the effects of bleeding at well points on cerebral edema in experimental ischemic rats after emergency treatment of cerebral lymphatic drainage block.Methods:Thirty male SD rats were randomized into the normal group,sham operation group,the model group,bleeding at well point group,ischemia+cervical lymphatic drainage block group and ischemia+cervical lymphatic drainage block+bleeding at well point group,Longa method and Casley-Smith method were used to establish pMCAO and CLB models respectively,to detect brain water content,brain coefficient value and brain density of the rats in different groups in six hours.Results:The model group was higher than bleeding at well point group in right brain water content,and the difference had statistical meaning(P<0.05),right brain water content of bleeding at well point group was less than that of ischemia+cervical lymphatic drainage block group and ischemia+cervical lymphatic drainage block+bleeding at well point group(P<0.05),there was no statistical difference between ischemia+cervical lymphatic drainage block group and ischemia+cervical lymphatic drainage block+bleeding at well point group(P>0.05).The difference had no statistical meaning among different groups in left brain coefficient(P>0.05);except the normal group and sham operation group,the difference showed statistical meaning in right brain coefficient when other groups were compared with the model group(P<0.05);the difference was statistically significant in whole brain coefficient when bleeding at well point group was compared with ischemia+cervical lymphatic drainage block group and ischemia+cervical lymphatic drainage block+bleeding at well point group(P<0.05),while the difference had no statistical meaning when ischemia+cervical lymphatic drainage block group was compared with ischemia+cervical lymphatic drainage block+bleeding at well point group(P>0.05).The difference showed statistical meaning when the model group,ischemia+cervical lymphatic drainage block group and ischemia+cervical l
作者
徐梦瑶
杜元灏
高靓
孟雪
XU Mengyao;DU Yuanhao;GAO Liang;MENG Xue(First Teaching Hospital of Tianjin University of TCM,Tianjin 300193,China;Tianjin University of Traditional Chinese Medicine)
出处
《西部中医药》
2021年第1期11-14,共4页
Western Journal of Traditional Chinese Medicine
基金
国家自然科学基金青年科学基金项目(81503634)。
关键词
脑缺血
脑水肿
脑淋巴引流阻滞
井穴放血
大鼠
cerebral ischemia
cerebral edema
brain lymphatic drainage block
bleeding at well point
rats
