摘要
为了探索外源重组白细胞介素17a(rIL-17a)对金黄色葡萄球菌感染的影响,试验在小鼠背部皮内注射金黄色葡萄球菌923与不同剂量(0.2μg、0.4μg、0.6μg、0.8μg、1.0μg)rIL-17a或牛血清白蛋白(BSA)的混合液,测定接种后不同时间点rIL-17a组和BSA组小鼠感染部位的伤口面积,接种6 d后血液、感染部位皮肤、肝脏、肾脏和脾脏内细菌数以及小鼠体重。结果表明:0.2μg rIL-17a与金黄色葡萄球菌混合皮内注射小鼠可极显著或显著减小感染第1,3天伤口面积(P<0.01或P<0.05),1.0μg rIL-17a可使感染第3天小鼠伤口面积显著增大(P<0.05)。感染6 d后,0.2μg和0.8μg rIL-17a组小鼠单位体积血液中的细菌数极显著低于相应剂量的BSA组(P<0.01),rIL-17a组和BSA组间皮肤内细菌数差异不显著(P>0.05),0.2μg rIL-17a组单位重量肝脏内细菌数极显著低于0.2μg BSA组(P<0.01),1.0μg rIL-17a组单位重量肝脏内细菌数则极显著高于1.0μg BSA组(P<0.01),1.0μg rIL-17a组单位重量肾脏内细菌数显著低于1.0μg BSA组(P<0.05),单位重量脾脏内细菌数在rIL-17a组和BSA组间的差异未达到统计学显著水平(P>0.05)。0.2μg和0.6μg rIL-17a组第5,6天可显著或极显著降低金黄色葡萄球菌对小鼠体重的抑制作用(P<0.05或P<0.01)。说明适当剂量的rIL-17a有助于皮肤感染处伤口愈合、血液和肝脏内金黄色葡萄球菌的清除、降低金黄色葡萄球菌对体重的影响;高剂量的rIL-17a不仅不利于清除细菌和促进伤口愈合,反而有利于细菌的生存和伤口恶化。
In order to explore the effect of exogenous recombinant interleukin 17 a(rIL-17 a) on Staphylocococcus aureus,a mixture of Staphylococcus aureus 923 and rIL-17 a(0.2 μg、0.4 μg、0.6 μg、0.8 μg、1.0 μg) or BSA were inoculated intracutaneously on the back of mice in this experiment. Wound sizes of the inoculation sites in rIL-17 a group and BSA group were measured at different time post inoculation,as well as bacteria counts in blood,inoculation sites,liver,kidney and spleen on 6 days post infection, and the body weights of mice were also calculated. The results showed that the mixture group of Staphylococcus aureus and 0.2 μg rIL-17 a inoculated intracutaneously could very significantly(P<0.01) and significantly(P<0.05) reduce wound sizes on day 1 and 3 post inoculation respectively. The mixture group with 1.0 μg rIL-17 a could increase significantly wound sizes of mice on day 3(P<0.05). On day 6 post inoculation,0.2 μg and 0.8 μg rIL-17 a groups had very significantly lower counts of Staphylococcus aureus than BSA group in mice blood per mL(P<0.01), rIL-17 a group and BSA group had no significant difference of bacteria counts in the inoculation sites(P>0.05),0.2 μg rIL-17 a group had very significantly lower bacteria counts than 0.2 μg BSA group in unit weight of liver(P<0.01). 1.0 μg rIL-17 a group had very significantly higher bacteria counts than 1.0 μg BSA group in unit weight of liver(P<0.01). 1.0 μg rIL-17 a group had significantly lower bacteria counts than 1.0 μg BSA group in unit weight of kidney(P<0.05). The rIL-17 a group and BSA group had no significant difference of bacteria counts in unit weight of spleen(P>0.05). In fifth and sixth day, 0.2 μg and 0.6 μg rIL-17 a groups could significantly(P<0.05) and very significantly(P<0.01), reduce the inhibitory effect of Staphylococcus aureus on mouce body weights respectively. The results suggested that an appropriate dose of rIL-17 a could be helpful to wound healing at the inoculation site,to the elimination of Staphylococcus aureus fr
作者
刘影
胡志秀
郭海勇
计银铎
代书玲
LIU Ying;HU Zhixiu;GUO Haiyong;JI Yinduo;DAI Shuling(Shanghai Vocational College of Agriculture and Forestry,Shanghai 201699,China;School of Life Scienc e,Jilin Normal University,Siping 136000,China;College of Veterinary Medicine,University of Minnesota,Twin City 55108,United States)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2021年第1期85-89,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
上海农林职业技术学院课题项目(KY2-0000-16-08)。
