摘要
目的:探讨油茶皂苷对血管瘤EOMA细胞的抑制作用。方法:选择小鼠血管瘤EOMA细胞作为实验对象,不同浓度油茶皂苷作用于EOMA细胞,采用CCK8法、AO染色及流式细胞术分别检测细胞增殖水平、细胞凋亡率及细胞周期分布。结果:油茶皂苷作用EOMA细胞24 h后,CCK8法检测油茶皂苷对EOMA细胞体外增殖能力有明显的抑制作用,且呈剂量依赖性。吖啶橙(AO)染色后荧光显微镜下观察可见细胞数量明显减少,细胞体积缩小,细胞核固缩,计数,与对照组相比,油茶皂苷20 mg·L^(-1)组、40 mg·L^(-1)组凋亡率分别为9.5%、14.0%,差异有统计学意义(P<0.05)。经流式细胞仪检测,与对照组相比,油茶皂苷20 mg·L^(-1)组、40 mg·L^(-1)组细胞G0/G1期比例增加而S期比例降低,差异均有统计学意义(P均<0.05)。结论:油茶皂苷可一定程度抑制血管瘤EOMA细胞的增殖,其作用机制可能是通过诱导EOMA细胞凋亡,将细胞阻滞于G0/G1期,抑制其增殖转化。
Objective:To investigate the inhibitory effect of sasanquasaponin on EOMA cells of hemangioma.Methods:Murine hemangioma EOMA cells were selected as experimental objects.Different concentrations of sasanquasaponin were used to act on EOMA cells.The changes of cell morphology after 24 hours of drug action by optical microscope were observed.CCK8 method was used to detect cell proliferation levels.Cells morphology and apoptosis rate were detected by AO staining.Flow cytometry was used to detect cell cycle distribution.Results:CCK8 method indicated that the proliferation ability of EOMA cells in vitro was obviously inhibited after 24 hours treatment with sasanquasaponin,and it was dose-dependent.When observing and counting apoptotic cells with fluorescence microscope after AO staining,the number of EOMA cells was reduced,the cell volume was reduced,and the nucleus was shrinking after 24 hours treatment with sasanquasaponin.The apoptosis rates of 20 mg·L^(-1) group and 40 mg·L^(-1) group were 9.5%and 14.0%,respectively,while the ratio of G0/G1 phase increased and the ratio of S phase decreased by Flow cytometer detection,and the differences were statistically significant(P<0.05).Conclusion:Sasanquasaponin inhibits the proliferation of hemangioma EOMA cells to a certain extent,and its mechanism may be promoting apoptosis and delaying the cell cycle in G0/G1 phase thus inhibiting their proliferation and transformation.
作者
周光华
谢琼珺
罗平
ZHOU Guang-hua;XIE Qiong-jun;LUO Ping(Department of Ophthalmology,The Fifth Affiliated Hospital of Southern Medical University,Guangzhou,Gaungdong 510900;School of Basic Medicine,Gannan Medical University,Ganzhou,Jiangxi 341000)
出处
《赣南医学院学报》
2021年第1期42-45,共4页
JOURNAL OF GANNAN MEDICAL UNIVERSITY
基金
广东省中医药局科研项目(编号:20182099)。
