摘要
目的探讨基质金属蛋白酶-13(MMP-13)、白细胞介素-17(IL-17)、核转录因子kppa B-p65(NF-κB-p65)在多发性骨髓瘤(MM)患者血清中的表达及其临床应用价值。方法选取2018年10月至2019年9月期间我院收治住院的86例MM患者作为研究对象,依据MM患者入院后的X线检查有无MM骨病,将患者划分为有MM骨病的MM骨病组(58例)和无MM骨病的MM组(28例);另选取于我院行健康体检正常的人群40例作为对照组,对MM患者及对照组的临床资料及血清MMP-13、IL-17、NF-κB-p65水平进行分析,探讨血清中MMP-13、IL-17、NF-κB-p65水平在MM及相关骨病临床诊断中的意义。结果MM骨病组和MM组血清MMP-13、IL-17、NF-κB-p65水平明显高于对照组,且MM骨病组以上三指标的血清表达水平均高于MM组(P均<0.001);单因素分析结果显示,MM骨病组与MM组在年龄、CRP水平、血清MMP-13、血清IL-17和血清NF-κB-p65水平方面存在明显差异(P<0.05);多因素回归分析结果显示,血清MMP-13≥7.5 pg/mL、血清IL-17≥30 pg/mL和血清NF-κB-p65≥2100 pg/mL是MM患者发生骨病的独立预测因素;ROC曲线分析结果显示,血清MMP-13、IL-17、NF-κB-p65水平联合预测MM骨病的AUC值明显高于三者单独诊断时的AUC值(P<0.05);经治疗后,依据MM患者治疗疗效划分为有效组(45例)和无效组(41例),发现有效组血清MMP-13、IL-17、NF-κB-p65水平均明显低于无效组(P<0.05)。结论血清MMP-13、IL-17、NF-κB-p65水平在MM及相关骨病患者血清中呈较高表达,血清三项指标联合检测对于临床上预测与诊断MM骨病具有较好的应用价值,且可能用于MM临床疗效的评估。
Objective To explore the expressions and clinical application values of serum matrix metalloproteinase-13(MMP-13),interleukin-17(IL-17)and nuclear transcription factor kppa B-p65(NF-κB-p65)in patients with multiple myeloma(MM).Methods A total of 86 MM patients who were admitted to the hospital from October,2018 to September,2019 were enrolled as the research objects.According to presence or absence of MM bone disease examined by X-ray after admission,they were divided into the MM bone disease group(58 cases)and MM group(28 cases).Another 40 healthy people who underwent physical examination in the hospital were enrolled as the control group.The clinical data,and levels of serum MMP-13,IL-17 and NF-κB-p65 in MM patients and the control group were analyzed.The significance of serum MMP-13,IL-17 and NF-κB-p65 levels in the clinical diagnosis of MM and related bone diseases was explored.Results The levels of serum MMP-13,IL-17 and NF-κB-p65 in the MM bone disease group and MM group were significantly higher than those in the control group.Also,these levels were higher in the MM bone disease group than the MM group(P<0.001).The results of univariate analysis showed that there were significant differences in age,levels of CRP,serum MMP-13,serum IL-17 and serum NF-κB-p65 between the MM bone disease group and MM group(P<0.05).The results of multivariate regression analysis showed that serum MMP-13 not lower than 7.5 pg/mL,IL-17 not lower than 30 pg/mL and NF-κB-p65 not lower than 2100 pg/mL were independent predictors of bone diseases in MM patients.The results of ROC curve analysis showed that the AUC of serum MMP-13 combined with IL-17 and NF-κB-p65 for predicting MM bone diseases was significantly higher than those of single marker tests(P<0.05).After treatment,according to different curative effect,patients were divided into the effective group(45 cases)and ineffective group(41 cases).The levels of serum MMP-13,IL-17 and NF-κB-p65 in the effective group were significantly lower than those in the ineffective gr
作者
邹夏
吴遥
刘蜀蓉
ZOU Xia;WU Yao;LIU Shurong(Department of Hematology,West China Hospital,Sichuan University,Chengdu 610000,China;Medical Rescue Center,Chengdu Shuangliu International Airport,Chengdu 610000,China)
出处
《标记免疫分析与临床》
CAS
2021年第2期262-267,293,共7页
Labeled Immunoassays and Clinical Medicine
