摘要
Antibiotic resistance represents a widespread problem in milk production.The identification of compounds for a topically applied ointment used in mastitis therapy remains elusive.Compounds from the genus Hymenaea can be administered in cases of multi-drugresistant Staphylococcus aureus infection for ruminant species,but the protective properties are not well known.Wi this research the aim is verify the protective effects of H.martiana against S.aureus infection in bovine mammary epithelial cell line(MAC-T)and to obtain an antioxidant profile evaluation in vitro.The MAC-T cells were challenged with S.aureus after being exposed to the extract of the H.martiana in the protective assay.For the verification of the viability of the MAC-T cells,the MTT assay was performed,and was used dilutions of the plant extract,starting at 2.5%.The extract of H.martiana was evaluated for antioxidant aspect in different dilutions by FRAP,ORAC and DPPH.A variety of flavonoids(quercetin,luteolin,etc.)have been identified as the main components by using mass spectrometry,reinforcing our in vitro findings that flavonoids,especially quercetin,have a medicinal profile capable of killing mastitis-causing bacteria.An excellent antioxidant pattern was observed in the 2.5%solution;however,membrane integrity in MAC-T cells was compromised.Those findings suggest low dilutions of H.martiana extract has a desirable protective effect from S.aureus pathogenesis.Our in vitro studies can be gleaned upon for further in vivo studies.
基金
The student was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil(CAPES)with the doctoral scholarship(2017)
The student has the support of the scholarship grant number 2017/22041-1,Sao Paulo Research Foundation(FAPESP)
The plant analysis is supported by the Facepe Pronem(APQ-0741106/2014)
We want to thank Dr.Magna Coroa Lima(UNIVIÇOSA/MG)for providing the bacteria from cases of caprine mastitis used in our study and Dr.Aberlado Silva Júnior(UFV/MG)for the opportunity to use the facilities.
