摘要
大豆是重要的植物蛋白质和植物油脂来源,干旱是影响大豆产量的重要环境因子之一。为解析大豆耐旱性的遗传基础,本研究在PEG水压胁迫条件下,对由409个家系组成的巢式关联作图群体(具有1个共同亲本的2个重组自交系群体组成)进行叶片脯氨酸含量测定,通过限制性二阶段多位点全基因组关联分析(restrictivetwo-stagemultilocus genome-wide association study,RTM-GWAS),解析了大豆根部水压胁迫耐逆指数(root hydraulic stress tolerance index,RHSTI)的遗传体系。结果表明,在春季和夏季环境下,3个亲本蒙8260(共同亲本)、通山薄皮黄豆甲和正阳白毛平顶在RHSTI上均存在显著差异,其衍生群体RHSTI表型变异丰富,变幅分别为0.11~2.94和0.03~1.93,遗传力分别为97.7%和97.9%;2个环境联合分析发现,家系遗传力和家系与环境互作遗传力分别为37.9%和60.1%,说明群体RHSTI的变异受遗传和环境共同控制。通过RTM-GWAS方法,共检测到45个与RHSTI相关的QTL,分布在大豆18条染色体上,可以解释37.58%的表型变异,其中7个QTL的表型贡献率超过1%,为大贡献位点;这些QTL中,有34个位点与环境存在显著互作,可以解释12.50%的表型变异。结合PEG胁迫下大豆转录组数据,在定位区间500kb范围内共筛选到38个差异表达基因,可归为ABA响应因子、逆境响应因子、转录因子、发育因子、蛋白代谢因子、未知功能和其他等7类,其中逆境响应因子、转录因子和发育因子是最大的3类;其中位于主效位点的6个基因,与ABA响应因子、逆境响应因子、转录因子相关,应为主要候选基因。上述结果表明,大豆耐旱性是一个由多位点、多基因控制的复杂数量性状,且与环境存在互作,遗传基础复杂。研究结果为大豆耐旱性分子育种提供了依据。
Soybean is an important source of plant protein and vegetable oil in the world.Drought is one of the important environmental stress factors affecting soybean yield.To explore the genetic base of drought tolerance in soybean,a nested association mapping population composed of two sets of recombinant inbred lines with a common parent in a total of 429 lines was investigated for leaf proline content under PEG simulated drought stress.The genetic system of root hydraulic stress tolerance index(RHSTI)was analyzed using the RTM-GWAS(restrictive two-stage multilocus genome-wide association study).The results showed that there were significant differences in RHSTI among the three parents under two different environments in spring and summer,and among the nested association mapping population with the variation range of 0.11–2.94 and 0.03–1.93,respectively.The heritability values of Line and Line×Environment were 37.9%and 60.1%,respectively,indicating that the variation of RHSTI was greatly affected by the environment.Using the RHSTI data and 6137 SNPLDB markers,a total of 45 main effect QTLs were detected on 18 chromosomes,which could explain a total of 37.58%phenotypic variation,including 7 large contribution QTLs with R2 more than 1%.Among them,34 QTLs with QTL×Environment effect explained 12.50%of the phenotypic variation.Combined with the transcriptome data under PEG stress,totally 38 differentially expressed genes were identified within a QTL±500 kb,which can be grouped into different biological categories,including ABA responders,stress responders,transcription factors,development factors,protein metabolism factors,unknown functions and others,with stress responses,transcription factors and development factors as the major parts.In summary,the results indicated that the drought tolerance of soybean was a complex quantitative trait,with the complex genetic basis controlling by multiple loci,multiple genes and interaction with the environment.The present results can lay the foundation of molecular breeding for
作者
王吴彬
童飞
KHAN Mueen Alam
张雅轩
贺建波
郝晓帅
邢光南
赵团结
盖钧镒
WANG Wu-Bin;TONG Fei;KHAN Mueen-Alam;ZHANG Ya-Xuan;HE Jian-Bo;HAO Xiao-Shuai;XING Guang-Nan;ZHAO Tuan-Jie;GAI Jun-Yi(Soybean Research Institute,Nanjing Agricultural University/National Center for Soybean Improvement/MOA Key Laboratory for Biology and Genetic Improvement of Soybean(General),Ministry of Agriculture and Rural Affairs/National Key Laboratory of Crop Genetics and Germplasm Enhancement/Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing 210095,Jiangsu,China;Xuzhou Institute of Agricultural Sciences of Xuhuai Region of Jiangsu,Xuzhou 221131,Jiangsu,China)
出处
《作物学报》
CAS
CSCD
北大核心
2021年第5期847-859,共13页
Acta Agronomica Sinica
基金
中央高校基本科研业务费专项(KYZZ201901)
国家自然科学基金项目(31601325)
教育部长江学者和创新团队项目(PCSIRT_17R55)
国家现代农业产业技术体系建设专项(CARS-04)
江苏省JCIC-MCP项目资助。
