摘要
目的:探究生肌化瘀方治疗慢性皮肤溃疡的作用机制。方法:利用TCMSP、TCMIP、TCMID、GeneCards、PubChem及SwissTargetPrediction数据库查找生肌化瘀方的化学成分并预测其潜在作用靶点,探索潜在作用靶点的生物学功能,利用Cytoscape软件构建"药物-化合物-靶点"多层次网络图,对网络的拓扑结构进行聚类分析,并对获得的靶点进行GO功能及KEGG通路分析。结果:生肌化瘀方共筛选得到845个化合物,并与1 095个靶标构成2 455个成分-靶点配对。生肌化瘀方的GO功能主要涉及代谢、跨膜转运、γ-氨基丁酸信号途径、炎症反应的负性调节等,KEGG通路主要涉及代谢途径、甾体激素生物合成、氮代谢、cAMP信号通路、氧化磷酸化、胰岛素抵抗、抗生素的生物合成、碳代谢、PPAR信号通路、胰岛素信号通路等。结论:生肌化瘀方可能通过多种成分和多个靶点对慢性皮肤溃疡发挥治疗作用,方中黄芪、大黄、血竭贡献较大。
Objective:To investigate the mechanisms of Shengji Huayu Formula in the treatment of chronic skin ulcer. Methods:The chemical components of Shengji Huayu Formula and its potential targets were found and predicted by TCMSP, TCMIP, TCMID, GeneCards, PubChem and SwissTargetPrediction databases. The biological functions of potential targets were explored. The multi-levels network graph of drug-compound-target was constructed by Cytoscape software, and the topological structure of the network was analyzed by cluster analysis. GO function and KEGG pathway analysis were performed for the obtained targets. Results:There were 845 compounds and 1 095 targets, which formed 2 455 component-target pairs. The GO function of Shengji Huayu Formula mainly involved metabolism, transmembrane transport, γ-aminobutyric acid signal pathway and negative regulation of inflammatory response, and KEGG pathway mainly included metabolic pathways, steroid hormone biosynthesis, nitrogen metabolism, cAMP signaling pathway, oxidative phosphorylation, insulin resistance, antibiotic biosynthesis, carbon metabolism, PPAR signaling pathway, insulin signaling pathway and so on. Conclusion:Shengji Huayu Formula may exert the therapeutic effects in treating chronic skin ulcer through multiple components and multiple targets, and Astragali Radix, Rhei Radix et Rhizoma and Draconis Sanguis play an important role.
作者
鲁冰
赵堃鹏
闫语
朱玮
李福伦
李斌
向延卫
LU Bing;ZHAO Kunpeng;YAN Yu;ZHU Wei;LI Fulun;LI Bin;XIANG Yanwei(Department of Deimatology,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China;Institute of Interdisciplinary Integrative Medicine Research,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Innovation Center of Traditional Chinese Medicine Health Service,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China)
出处
《上海中医药大学学报》
CAS
2021年第1期102-111,共10页
Academic Journal of Shanghai University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81673866)
国家重点研发计划“中医药现代化研究”重点专项(2018YFC1705300)。
关键词
生肌化瘀方
创面愈合
网络药理学
Shengji Huayu Formula
wound healing
network pharmacology
