摘要
目的探讨乙二胺四乙酸二钠(ethylenediaminetetraacetic acid disodium,EDTA-2Na)联合抗菌药物对铜绿假单胞菌的体外抑菌及生物膜抑制作用。方法对临床分离的10株亚胺培南耐药铜绿假单胞菌、8株黏菌素耐药铜绿假单胞菌和8株阿米卡星耐药铜绿假单胞菌,通过微量肉汤稀释棋盘法检测EDTA-2Na分别与亚胺培南、黏菌素和阿米卡星联用以及各自单用时对相应耐药菌株的最小抑菌浓度(minimal inhibititory concentration,MIC),并通过计算部分抑菌浓度指数(fractional inhibitory concentrationindex,FICI)评价联合抑菌效果;结晶紫生物膜试验检测EDTA-2Na分别与亚胺培南、黏菌素和阿米卡星联用及各自单用时对相应耐药菌株生物膜的抑制作用,通过测量A595值评价抑制生物膜效果。结果EDTA-2Na与亚胺培南、黏菌素和阿米卡星联用后对相应耐药菌株的抑菌作用表现为协同或相加,对生物膜也具有明显的协同抑制作用。结论EDTA-2Na联合抗菌药物对铜绿假单胞菌具有良好的体外抑菌作用及抑制生物膜作用。
Objective To evaluate the inhibitory and biofilm inhibitory effects of ethylenediaminetetraacetic acid disodium(EDTA-2 Na)combined with antibacterial agents against Pseudomonas aeruginosa isolates in vitro.Methods Ten clinical isolates of imipenem-resistant P.aeruginosa,eight colistin-resistant P.aeruginosa isolates,and eight amikacin-resistant P.aeruginosa isolates were collected from different specimens of different inpatients from the First Affiliated Hospital of Wenzhou Medical University from 2015 to 2017.Minimal inhibitory concentrations(MICs)of EDTA-2 Na combined with imipenem,colistin or amikacin,and the four drugs used alone against P.aeruginosa were detected by the checkerboard dilution method,and the fractional inhibitory concentration index(FICI)was calculated to evaluate antibiotic effects.The inhibitory effect of EDTA-2 Na combined with imipenem,colistin or amikacin,and the latter three drugs used alone against P.aeruginosa were detected by the crystal violet biofilm test,and the A595 values were measured to evaluate biofilm inhibitory effects.Results The inhibitory effects of the combinations of EDTA-2 Na with imipenem,colistin or amikacin separately for the antibiotic-resistant P.aeruginosa isolates were synergistic or additional,and no effects of uncorrelated and antagonism were observed in all these combinations.The biofilm inhibitory effects of the combinations of EDTA-2 Na with imipenem,colistin or amikacin separately for the antibiotic-resistant P.aeruginosa isolates were synergistic.Conclusions EDTA-2 Na combined with antibacterial agents can improve the inhibitory and the biofilm inhibitory effects for P.aeruginosa in vitro.
作者
廖文丽
孙瑶
陈涛
喻凯航
徐雯雅
林婕
周铁丽
Liao Wen-li;Sun Yao;Chen Tao;Yu Kai-hang;Xu Wen-ya;Lin Jie;Zhou Tie-li(Laboratory Medicine Center,the First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325000;School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou 325000)
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2021年第2期137-142,共6页
Chinese Journal of Antibiotics
基金
国家自然科学基金(No.81971986)
浙江省教育厅一般科研项目(No.Y201942275)。
