摘要
目的探讨长链非编码RNA(LncRNA)基因在幼羊颅骨缺损修复的差异性表达及改善颅骨缺损的作用。方法将12只1月龄的小尾寒羊分成2组:原生骨组织组、新生骨组织组,每组6只。通过高通量测序检测两组骨组织内LncRNA的表达变化,通过Real-time PCR验证测序LncRNA表达量的变化。ELISA法检测血清中碱性磷酸酶(ALP)和钙调蛋白(OCN);Western blot检测Wnt/β-catenin的蛋白表达水平。结果与原生骨组织组相比,新生骨组织中LncRNA ODSM、LncRNA UCA1表达上调,LncRNA H19、LncRNA MEG3的表达下调;新生骨组织组幼羊血清中ALP、OCN的含量升高;新生骨内的Wnt/β蛋白的表达升高。结论 LncRNA ODSM、UCA1可能调控Wnt/β信号通路参与缺损部位颅骨组织的修复。
Objective To investigate the differential expression of long noncoding RNA(LncRNA) gene in the regeneration of skull defects in young sheep and its effects in improving skull defects. Methods Twelve 1-month-old small-tailed Han sheep were divided into the primary bone tissue group and the skull defect group, with 6 in each group. High-throughput sequencing was used to detect the expression changes of LncRNA in the two groups of bone tissues, and Real-time PCR was used to verify the changes in the expression of sequencing LncRNA. The serum alkaline phosphatase(ALP) and osteocalcin(OCN) were detected by ELISA. The protein expression levels of Wnt and β-catenin were detected by Western blot. Results The expressions of LncRNA ODSM and LncRNA UCA1 were up-regulated, while the expressions of LncRNA H19 and LncRNA MEG3 were down-regulated in the new bone tissue;the content of ALP and OCN in serum of the new bone group increased;the expression of Wnt/β protein in bone increased compared with the original bone tissue group. Conclusion LncRNA ODSM and UCA1 may regulate Wnt/β signaling pathway and participate in the regeneration of skull tissue at the defect site.
作者
刘岩
邵国
张春阳
Liu Yan;Shao Guo;Zhang Chunyang(Dept of Neurosurgery,The First Affiliated Hospital of Baotou Medical College,Inner Mongolia University of Science and Technology,Baotou 014040;Institute of Neurosurgical Diseases of Baotou Medical College,Translational Medicine,Inner Mongolia University of Science and Technology,Baotou 014040;Engineering Technology Center for Bone Tissue Regeneration and Injury Repair in Inner Mongolia Autonomous Region,Baotou 014040)
出处
《安徽医科大学学报》
CAS
北大核心
2021年第1期82-86,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81660214、81960238)。
