摘要
目的探索孕妇口腔菌群结构和多样性及其影响因素,识别孕期口腔微生态核心菌群。方法对安庆市立医院产科门诊2018年5月11日-7月12日行产检的100例健康孕妇采集口腔样本并进行问卷调查,扩增样本中16S rRNA的V3~V4片段并进行测序,利用QIIME 2与R软件分析孕妇菌群结构和丰度,通过LEfSe(LDA Effect Size)进行组间差异菌群分析,在Usearch 11软件中对样品中OTU(operational taxonomic units)出现频率进行排序,筛选核心菌群。结果测序共获得用于物种分类的OTU 884个,涵盖了13门、21纲、49目、85科、157属的细菌。孕妇口腔菌群相对丰度前三位依次为链球菌属Streptococcus(33.6%)、窄食单胞菌属Stenotrophomonas(12.4%)、普氏菌属Prevotella(7.5%)。不同年龄、孕周及牙周健康情况孕妇的口腔菌群多样性差异无统计学意义(P> 0.05)。年龄组间显著差异菌群为颗粒链球菌属(Granulicatella)与二氧化碳嗜纤维菌属(Capnocytophaga),富集于年龄> 35岁的高龄孕妇口腔中;不同孕周组间未见显著差异菌群;牙周健康组与牙周疾病组显著差异菌群有奈瑟菌属和劳特罗普氏菌属(Lautropia),富集于牙周健康组。结论孕妇口腔菌群核心菌群包括链球菌属、窄食单胞菌属、普氏菌属等;其多样性较为稳定,颗粒链球菌属与二氧化碳嗜纤维菌属丰度随年龄增加而升高;牙周健康者奈瑟菌属和劳特罗普氏菌属丰度较高。
Objective To explore the structure and diversity of oral microbiota in pregnant women and its influencing factors,and identify the core microbiota in oral cavity during pregnancy. Methods One hundred samples from healthy pregnant women screened from May 11 to July 12,2018 in the obstetrics clinic of Anqing Municipal Hospital were collected and investigated.The V3-V4 regions of 16S rRNA in the samples were amplified and sequenced. The structure and abundance of pregnant women’s oral microbiota were compared using QIIME 2 and R. The differential microbiota between groups was recognized by LDA Effect Size(LEfSe). The frequencies of operational taxonomic units(OTU) in the samples were sorted by Usearch 11 to identify the potential core microbiota. Results A total of 884 OTUs were sequenced with a coverage of 13 species,21 phyla,49 orders,85 families,and 157 genera. The oral microbiota with highest relative abundance in pregnant women were Streptococcus(33. 6%),Stenotrophomonas(12. 4%),Prevotella(7. 5%) and Rothia(6. 3%),Haemophilus(4. 6%),Leptorichia(4. 0%),Neisseria(3. 0%),Veillonella(2. 7%),Porphyromonas(2. 6%),and Fusobacterium(2. 3%). There was no statistical significance on the difference in the diversity of oral microbiota among pregnant women of different ages,gestational weeks and periodontal conditions(P > 0. 05). Granulicatella and Capnocytophaga were found enriched in the oral cavity of women aged over 35 years,and there was no statistical significance on the differential microbiota among women with different gestations. The significant differential microbiota among the periodontal conditions were Neisseria and Lautropia,which were enriched in the periodontal normal group. Conclusion The core microbiota of pregnant women includes Streptococcus,Stenotrophomonas,Prevotella,Rothia,Haemophilus,Leptorichia,Neisseria,Veillonella,Porphyromonas,and Fusobacterium. Diversity of oral microbiota in pregnant women is relatively stable,and the abundance of Granulicatella and Capnocytophaga increases with age. The prop
作者
王天雷
李青
刘海燕
李飞健
胡安群
郑英杰
WANG Tian-lei;LI Qing;LIU Hai-yan;LI Fei-jian;HU An-qun;ZHENG Ying-jie(Department of Epidemiology,School of Public Health,Fudan University,Shanghai 200032,China;不详)
出处
《中国卫生检验杂志》
CAS
2021年第2期129-133,共5页
Chinese Journal of Health Laboratory Technology
基金
国家自然科学基金(81373065,81773490)
国家重点研发计划“生物安全关键技术研发”重点专项(2017YFC-1200203)。
