摘要
目的探讨经典型骨髓增殖性肿瘤(MPN)患者血清炎症相关细胞因子[肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-1β、白细胞介素2受体(IL-2R)、IL-6、IL-8、IL-10]水平及其临床意义。方法选取50例费城染色体(Ph)阴性(Ph-)的初诊MPN患者[MPN组,其中真性红细胞增多症(PV)16例、原发性血小板增多症(ET)25例、原发性骨髓纤维化(PMF)9例],以20名体检健康者作为正常对照组,采用化学发光法检测血清TNF-α、IL-1β、IL-2R、IL-6、IL-8及IL-10水平,采用Sanger测序法检测MPN患者JAK2及MPL基因突变,采用实时荧光聚合酶链反应(PCR)检测CALR基因突变。结果MPN组血清TNF-α、IL-2R、IL-6和IL-8水平均显著高于正常对照组(P<0.001),血清IL-10水平显著低于正常对照组(P<0.001)。2个组之间血清IL-1β水平差异无统计学意义(P>0.05)。PMF组血清IL-2R水平高于PV组和ET组(P<0.05)。在JAK2 V617F突变阳性患者中,PMF组血清IL-2R水平显著高于PV组和ET组(P<0.01),血清TNF-α和IL-8水平高于ET组(P<0.05),血清IL-6水平高于PV组(P<0.05)。骨髓纤维化分级为MF-3级的MPN患者IL-2R水平高于MF-2级患者(P<0.05),TNF-α水平高于MF-0级患者(P<0.05)。结论MPN患者炎症相关细胞因子(TNF-α、IL-1β、IL-2R、IL-6、IL-8及IL-10)水平异常,且在3种MPN亚型之间也存在差异,或许可作为MPN诊断分型及随访的辅助参考指标。
Objective To investigate serum levels of inflammatory cytokines in patients with classical myeloproliferative neoplasms(MPN)[tumor necrosis factor-alpha(TNF-α),interleukin-1 beta(IL-1β),interleukin-2 receptor(IL-2R),interleukin-6(IL-6),interleukin-8(IL-8),interleukin-10(IL-10)]and their clinical significance.Methods The serum levels of TNF-α,IL-1β,IL-2R,IL-6,IL-8 and IL-10 were detected by chemiluminescence immunoassay(CLIA)in 50 patients with Philadelphia chromosome-negative(Ph-)newly diagnosed MPN[MPN group,including 16 cases of polycythemia vera(PV),25 cases of essential thrombocytosis(ET)and 9 cases of primary myelofibrosis(PMF)]and 20 healthy volunteers(control group).Sanger sequencing was used to detect JAK2 and MPL mutations,and CALR mutation was detected by real-time fluorescent polymerase chain reaction(PCR)in MPN patients.Results The serum levels of TNF-α,IL-2R,IL-6 and IL-8 in MPN group were significantly higher than those in control group(P<0.001),while IL-10 level was on the contrary(P<0.001).There was no significant difference in serum IL-1βlevel between the two groups(P>0.05).IL-2R level in PMF group was higher than that in PV and ET groups(P<0.05).Among the JAK2 V617F mutation-positive patients,IL-2R level in the PMF group was also significantly higher than that in the PV and ET groups(P<0.01);the levels of TNF-αand IL-8 in the PMF group were higher than those in the ET group(P<0.05);the level of IL-6 in the PMF group was higher than that of the PV group(P<0.05).Interestingly,the level of TNF-αin MPN patients with marrow fibrosis(MF)-3 was higher than that in patients with MF-0(P<0.05)and its IL-2R level was higher than that of patients with MF-2(P<0.05).Conclusion Inflammatory cytokines(TNF-α,IL-1β,IL-2R,IL-6,IL-8 and IL-10)are abnormally expressed in MPN patients,and there are differences in the expression among the three subtypes of MPN.Cytokine levels might become potential reference indicator for MPN diagnostic classification and follow-up.
作者
陈朴
马艳婷
陈楠
于正麟
程韵枫
潘柏申
郭玮
王蓓丽
CHEN Pu;MA Yangting;CHEN Nan;YU Zhenglin;CHENG Yunfeng;PAN Boshen;GUO Wei;WANG Beili(Department of Laboratory Medicine,Zhongshan Hospital,Fudan University,Shanghai 200032,China;Department of Hematology,Zhongshan Hospital,Fudan University,Shanghai 200032,China)
出处
《检验医学》
CAS
2021年第2期167-172,共6页
Laboratory Medicine
基金
国家自然科学基金面上项目(81572064)
国家自然科学基金面上项目(81772263)
上海市卫生计生系统重要薄弱学科建设项目(2015ZB0201)
上海市卫生和计划生育委员会资助项目(201540052)
上海市卫生和计划生育委员会资助项目(201440389)
上海市科学技术委员会资助项目(16411952100)。
