摘要
目的分析血清微小RNA-152(miR-152)检测在非肌层浸润性膀胱癌(NMIBC)诊断和复发预测中的价值。方法前瞻性选取2016年6月至2017年6月航天中心医院收治的80例NMIBC患者作为病例组,选取同期80名接受体检者作为对照组,对2组研究对象的血清miR-152相对表达量进行检测,分析其对NMIBC的诊断价值;对病例组患者进行36个月的术后随访,对其术后复发情况进行随访,分析血清miR-152相对表达量与NMIBC术后复发的相关性及对复发的预测价值。结果在随访期内有9例患者失访,病例组最终纳入71例。病例组患者的血清miR-152相对表达量低于对照组(0.50±0.36 vs.1.15±0.65),差异有统计学意义(P<0.05)。在随访期内,有18例患者发生复发,复发率为24.32%。复发组患者的肿瘤分期为T1期、多发性肿瘤、肿瘤低分化的比例高于未复发组,血清miR-152相对表达量低于未复发组(0.24±0.22 vs.0.59±0.35),差异均有统计学意义(P<0.05)。NMIBC术后复发与血清miR-152相对表达量、肿瘤数目、分化程度具有相关性(P<0.05)。血清miR-152相对表达量诊断NMIBC和预测NMBIC术后复发的受试者工作特征曲线下面积(AUC ROC)均具有统计学意义(P<0.05),在Cut off值下,其敏感度和特异度分别为0.688、0.873和0.755、0.833。结论在NMIBC患者可观察到血清miR-152表达下调,且其相对表达量的下降与患者的术后复发风险有关,可作为辅助NMIBC诊断和复发预测的血清标志物。
Objective To analyze the values of serum microRNA(miR)-152 in the diagnosis and recurrence prediction of non muscle invasive bladder cancer(NMIBC).Methods From June 2016 to June 2017,80 cases of NMIBC patients admitted to the Aerospace Center Hospital were selected prospectively as the case group,and 80 physical examinees in the same period were selected as the control group.The relative expression of miR-152 in the serum of the patients in the two groups were detected,and its diagnostic value for NMIBC was analyzed;the patients in the case group were followed up for 36 months,and the recurrence rate was followed up,the correlation between relative expression of serum miR-152 and the recurrence and its predictive value for recurrence were analyzed.Results During the follow-up period,9 cases were lost to follow-up,and 71 cases were finally included in the case group.The relative expression of miR-152 in serum of the patients in the case group was lower than that in the control group(0.50±0.36 vs.1.15±0.65),the difference was statistically significant(P<0.05).During the follow-up period,18 patients recurred,the recurrence rate was 24.32%.The proportions of T1 stage,multiple tumor and poorly differentiated tumor of the patients in the recurrence group were higher than those in the non recurrence group,and the relative expression of miR-152 in serum was lower than that in the non recurrence group(0.24±0.22 vs.0.59±0.35),the differences were statistically significant(P<0.05).The recurrence of NMIBC after operation was correlated with the relative expression of miR-152,the number of tumor and the degree of differentiation(P<0.05).The area under the receiver operating characteristic curve(AUC ROC)of serum miR-152 relative expression in diagnosis of NMIBC and prediction of recurrence after NMIBC operation were statistically significant(P<0.05).Under the cut off values,the sensitivities and specificities were 0.688,0.873 and 0.755,0.833,respectively.Conclusion Down-regulation of serum miR-152 expression can be observe
作者
徐峰
杨勇
刘忠平
付斌
徐震
XU Feng;YANG Yong;LIU Zhong-ping(Department of Urology,Aerospace Center Hospital,Beijing 100049,China;Department of Urology,Fourth Medical Center,General Hospital of Chinese PLA,Beijing 100037,China.)
出处
《临床和实验医学杂志》
2021年第3期289-293,共5页
Journal of Clinical and Experimental Medicine
基金
北京市科技计划项目(编号:2016010214)。
