摘要
目的探讨FoxF1和FoxF2转录因子通过抑制p21Cip1 CDK调控早期胃癌能量代谢的机制。方法将对数生长期的HGC27细胞分为FoxF1组、FoxF2组和对照组,分别转染200 ng/mL pcDNA3.0-FoxF1、200 ng/mL pcDNA3.0-FoxF2和等体积的磷酸盐缓冲液,采用CCK法检测细胞增殖,流式细胞仪检测细胞凋亡,Transwell小室检测细胞迁移,蛋白质印迹检测蛋白表达,比色法检测葡萄糖、乳酸水平。结果转染24、36 h后,FoxF1组和FoxF2组的细胞增殖指数、迁移指数、p21Cip1、CDK1蛋白相对表达水平、葡萄糖、乳酸水平均显著低于对照组,差异有统计学意义(P<0.05),细胞凋亡指数、FoxF1和FoxF2蛋白相对表达量均显著高于对照组,差异有统计学意义(P<0.05),而FoxF1组和FoxF2组相比以上指标差异均无统计学意义(P>0.05)。结论FoxF1和FoxF2转录因子的高表达能抑制p21Cip1/CDK信号通路的激活,导致糖酵解水平降低,从而抑制细胞增殖与迁移,促进细胞凋亡。
Objective To investigate the mechanism by which FoxF1 and FoxF2 transcription factors regulate energy metabolism in early gastric cancer by inhibiting p21Cip1 CDK.Methods HGC27 cells in the logarithmic growth phase were divided into FoxF1 group,FoxF2 group and control group.The three groups were transfected with 200 ng/mL pcDNA3.0-FoxF1,200 ng/mL pcDNA3.0-FoxF2 and equal volume of phosphate buffer.CCK method were used to detect cell proliferation,flow cytometry to detect cell apoptosis,Transwell chamber to detect cell migration,Western blotting to detect protein expression,and colorimetric method to detect glucose and lactate levels.Results At 24 h and 36 h after transfection,the cell proliferation index,migration index,relative expression levels of p21Cip1,CDK1 protein,glucose,and lactate levels of FoxF1 and FoxF2 groups were lower than those of the control group(P<0.05).The apoptosis index and the relative expression of FoxF1 and FoxF2 proteins were significantly higher than those of the control group(P<0.05),but there was no statistically significant difference in the above indicators between the two groups(P>0.05).Conclusion The high expression of FoxF1 and FoxF2 transcription factors can inhibit the activation of p21Cip1/CDK signaling pathway,resulting in a decrease in glycolysis level,thereby inhibiting cell proliferation and migration,and promoting cell apoptosis.
作者
鲁昌坤
袁胜春
吴艳秋
LU Chang-kun;YUAN Sheng-chun;WU Yan-qiu(Department of Gastroenterology,Liaoyang Central Hospital,Liaoyang Liaoning 111000,China.;Department of Pathology,Liaoyang Central Hospital,Liaoyang Liaoning 111000,China.)
出处
《临床和实验医学杂志》
2021年第3期245-248,共4页
Journal of Clinical and Experimental Medicine
基金
国家卫生计生委医药卫生科技发展研究中心项目(编号:W2015CAE173)。
