摘要
为了探究植物激素变化与百合休眠和花芽分化之间的关系,以’木门’百合为试材,通过糖类物质变化和显微观测确定了休眠解除和花芽分化时期,并通过HPLC-MS技术进行了激素含量变化分析。结果表明’木门’百合种球在3℃冷藏条件下56天左右休眠解除,70天左右开始花芽分化,其进程分为花芽分化初期、小花原基分化期和花器官分化期3个时期;休眠解除前GA3/ABA上升,IAA/ABA、ZR/ABA比值保持低水平,休眠解除后GA3/ABA保持升高,IAA/ABA、ZR/ABA比值升高;花芽分化初期ZR上升、GA3下降、ZR/GA3急剧上升,小花原基分化期ZR下降、GA3上升、ZR/GA3急剧下降,花器官分化期ZR和GA3均上升,ZR/GA3稳定不变。由此可见,低水平IAA/ABA、ZR/ABA促进休眠维持,持续上升的GA3/ABA促进休眠解除,ZR/GA3上升促进花芽分化起始,下降则促进小花原基形成。本研究结果将为’木门’百合的采后储藏和开花调节提供一定理论参考。
To explore the relationship between hormone change and dormancy and flower bud differentiation of lily,Lilium oriental×trumpet hybrid’ConcaD’Or’was used as material,the stages of dormancy release and flower bud differentiation were identified according to carbohydrate change and microscopic observation,and the changes of hormones were analyzed by HPLC-MS technique.The results showed that the bulb dormancy was released after about 56 days at cold storage of 3℃,and then entered flower bud differentiation process at about 70 days,which was divided into three stages:flower bud differentiation initial stage,floret primordium differentiation stage and flower organ differentiation stage.Before dormancy release,GA3/ABA increased,IAA/ABA and ZR/ABA remained at low level.After dormancy release,GA3/ABA remained rising,IAA/ABA and ZR/ABA increased.At the initial stage of flower bud differentiation,the content of ZR increased,the content of GA3 decreased and ZR/GA3 went up sharply.At the stage of floret primordium differentiation,the content of ZR decreased,the content of GA3 increased and ZR/GA3 went down sharply.At the stage of flower organs differentiation,the contents of ZR and GA3 showed an upward trend and ZR/GA3 remained stable.These results indicated that low level of IAA/ABA and ZR/ABA promoted dormancy maintenance,continuous increase of GA3/ABA promoted dormancy release,the rising of ZR/GA3 promoted the initiation of flower bud differentiation,while its decline promoted the formation of floret primordium.The results will provide references for postharvest storage and flowering regulation of Lilium oriental×trumpet hybrid’ConcaD’Or’.
作者
李心
杨柳燕
陈敏敏
王桢
许俊旭
杨贞
张永春
Li Xin;Yang Liuyan;Chen Minmin;Wang Zhen;Xu Junxu;Yang Zhen;Zhang Yongchun(Forestry&Fruit Tree Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201403)
出处
《中国农学通报》
2020年第35期42-47,共6页
Chinese Agricultural Science Bulletin
基金
上海市科技兴农重点攻关项目“百合、红掌等花器官发育与调控的生理机制研究”(沪农科攻字(2014)第7-2-3号)。
