摘要
目的:研究并完善藜芦的质量控制。方法:采用薄层色谱法鉴别藜芦;采用HPLC法同时测定藜芦中虎杖苷和白藜芦醇的含量,采用Thermo C18色谱柱(4.6 mm×250 mm 5μm),以乙腈为流动相A,以水为流动相B,进行梯度洗脱流速1.0 mL/min;检测波长306 nm,柱温30℃。结果:薄层色谱斑点清晰分离度较好;虎杖苷和白藜芦醇分别在0.2004~2.004μg、0.4056~4.0563μg范围内呈现良好的线性关系,加样回收率分别为99.6%和100.1%,RSD分别为1.1%、1.0%。结论:该方法操作简单,准确度高,灵敏度高,重现性好,专属性强,可用于藜芦的质量控制。
Objective To study and improve the quality of Veratrum.Methods TLC was used to identify Veratrum;The Polydatin and Resveratrol was eluted on Thermo C18(250 mm×4.6 mm,5μm)with mobile phase of acetonitrile(A)-water(B)for gradient elution at the column temperature of 30℃.The flow rate of 1.0 mL/min and the detection wavelength was 306 nm.Results The TLC spots were clear and showed a good separation;Polydatin and Resveratrol showed good linear relationships in the ranges of 0.2004~2.004μg and 0.4056~4.0563μg respectively.The sample recovery rates were 99.6%and 100.1%,the RSDs were 1.1%and 1.0%.Conclusion The method is simple,accurate,high sensitivity,good reproducibility,high specificity,and can be used for quality control of Veratrum.
作者
程韬
王影超
陈韦佳
苌玲
舒柯
CHENG Tao;WANG Yingchao;CHEN Weijia;CHANG Ling;SHU ke(Guizhou Institute For Food And Drugs Control,Guiyang 550004,China;Guizhou Medical Unversity,Guiyang 550025,China)
出处
《中国民族民间医药》
2021年第2期29-32,共4页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
贵州省中药材、民族药材质量标准研究课题(DBYC52279)。
