摘要
运用胶体金免疫层析法与酶抑制率法快速检测蔬菜、水果中高风险农药残留,以腈菌唑、三唑磷、水胺硫磷、氟虫腈、百菌清5种农药为例。结果显示,胶体金免疫层析法重复性好,有较高的特异性和灵敏度,检出限可满足GB 2763—2019中规定的最大残留限量要求,无假阳性、假阴性现象,相对准确度较高。与现行有效的国家标准GB/T 5009.199—2003中酶抑制率法相比,胶体金试纸条具有操作简便、检测速度快、受检测环境温度影响较小等特点,适用于对蔬菜、水果中农药残留的现场筛查和检测。
Colloidal gold immunochromatography assay and enzyme inhibition methods were used to rapidly detect high risk pesticide residues in vegetables and fruits.Five pesticides including myclobutanil,triazophos,isocarbophos,fipronil and chlorothalonil were taken as examples.The results showed that the colloidal gold immunochromatography assay had better repeatability,higher specificity and higher sensitivity,and the detection limit could meet the maximum residue limit specified in GB 2763-2019.Furthermore,neither false-positive and false-negative test results were obtained using this assay,also relative accuracy was higher.Compared with enzyme inhibition method according to the current national standard GB/T 5009.199-2003,the colloidal gold immunochromatography assay had the characteristics of simple operation,fast detection,and was less affected by ambient temperature,and was suitable for on-site screening and detection of pesticide residues in vegetables and fruits.
作者
邓家军
杨琳芬
张富生
王兆芹
廖健
魏敏芝
叶琴
王茜
张志芳
陈玲
DENG Jia-jun;YANG Lin-fen;ZHANG Fu-sheng(Testing Center of Agro-product Quality and Safety of Jiangxi Province,Nanchang,Jiangxi 330046;Laboratory of Quality and Safety Risk Assessment for Agro-products of Ministry of Agriculture and Rural Affairs(Nanchang),Nanchang,Jiangxi 330046)
出处
《安徽农业科学》
CAS
2021年第4期191-197,共7页
Journal of Anhui Agricultural Sciences
基金
江西省蔬菜质量安全风险评估项目(30204003)。
关键词
胶体金免疫层析法
酶抑制率法
快速检测
果蔬
农药残留
Colloidal gold immunochromatography assay
Enzyme inhibition method
Rapid detection
Vegetables and fruits
Pesticide residues
