摘要
目的掌握广州地区禽类市场H6N2亚型禽流感病毒的分子遗传特征,为禽流感的科学防控提供研究数据。方法对禽类市场外环境分离株A/EN/Guangzhou/13565/2018(H6N2)进行全基因组序列测定,应用生物信息软件分析分子遗传特征。结果A/EN/Guangzhou/13565/2018(H6N2)毒株各基因片段最大相似度毒株均为国内H6N2禽流感病毒,核苷酸最大相似性在96.85%~98.22%之间,氨基酸最大相似性在96.30%~99.30%之间。各基因片段均属于欧亚谱系,HA、PB2、PB1、PA、M、NS基因归属于ST339-like分支,NP基因归属于HN573-like分支,发生多分支进化来源的重组。NA基因在HxN2亚型基因中归属于H6N2分支。该病毒为禽源低致病性病毒,但在PB2-89位、PB1-473位、PB1-F2蛋白、PA-409位、M1-30/215位、NS1-42/205位均发生对哺乳动物致病性增强的氨基酸变异。结论广州地区禽类市场监测发现的1株重组H6N2禽流感病毒,属于禽源低致病性病毒,但存在发生跨宿主传播的潜在风险,需加强监测。
In this study,the molecular characteristics of H6N2 subtype avian influenza virus isolated in a poultry market in Guangzhou were analyzed,to provide research data for scientific prevention and control of avian influenza.The whole genome of A/EN/Guangzhou/13565/2018(H6N2)was sequenced.The molecular characteristics were analyzed with bioinformatics software.The most similar strains of each gene segment of this isolate were strains of the H6N2 avian influenza virus isolated in China.The maximum nucleotide sequence similarity ranged from 96.85%to 98.22%,and that of amino acids ranged from 96.30%to 99.30%.All gene fragments belonged to the Eurasian lineage.The genes HA,PB2,PB1,PA,M and NS belonged to the ST339-like branch,whereas the NP gene belonged to the HN573-like branch,thus suggesting that this isolate recombined from strains from multiple evolutionary sources.The NA gene belonged to the H6N2 subtype avian influenza viruses rather than the H1N2,H3N2,H4N2,H5N2 or H9N2 subtypes.This isolate was an avian-origin virus with low pathogenicity.However,amino acid variations were found at the PB2-89,PB1-473,PB1-F2,PA-409,M1-30/215 and NS1-42/205 sites,which enhance the pathogenicity to mammals.In conclusion,a recombinant H6N2 subtype avian influenza virus,detected in the poultry market in Guangzhou,was found to be an avian-origin virus with low pathogenicity.Because of the potential risk that this strain might infect mammals,continuous monitoring of H6N2 subtype avian influenza virus is needed.
作者
曹蓝
夏丹
刘艳慧
曾庆
陈艺韵
陆剑云
李魁彪
张周斌
狄飚
CAO Lan;XIA Dan;LIU Yan-hui;ZENG Qing;CHEN Yi-yun;LU Jian-yun;LI Kui-biao;ZHANG Zhou-bin;DI Biao(Guangzhou Center for Disease Control and Prevention,Guangzhou 510440,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2021年第1期91-98,共8页
Chinese Journal of Zoonoses
基金
广东省自然科学基金(No.2019A1515011510、No.2019A1515011407)
广州市卫生和计划生育科技项目(No.20201A011052、No.20191A011064、No.20201A011067)
广东省医学科学技术研究基金(No.A2019149、No.A2019379、No.A2020399)
广州市医学重点学科建设项目(No.2017-2019-07)
国家科技重大专项(No.2017ZX10103011-005)联合资助。
关键词
禽流感病毒
H6N2
基因重组
变异
avian influenza virus
H6N2
genetic recombination
variation
