摘要
当归提前抽薹严重影响产量,而当归抽薹分子遗传机理尚不清楚。本研究利用RNA-seq技术对连续五代抽薹当归的叶片、茎段、种子、根进行测序,对所有组装后的Unigenes进行功能注释,寻找调控当归抽薹主要候选基因。结果表明:在当归的叶片、根、种子、茎段转录本中分别得到7.1、7.63、7.23、6.59 Gb数据,通过Nr、Nt、Swissprot、KEGG、KOG、Interpro、GO、Intersection、Overall等蛋白数据库进行蛋白质预测,分别预测了71 024、59 931、49 694、51 832、55 320、46 908、35 598、17 133、79 161个蛋白。所有转录本中80.13%预测蛋白与胡萝卜同源。在KEGG通路中共有2 666个差异表达的基因参与植物信号传导;与拟南芥、胡萝卜、芥菜中参与调控开花相关基因比对,共筛选出56条候选基因,将56条候选基因在NCBI中进行核酸比对,得到与直接控制抽薹AP1转录因子同源的Unigene45683_All、激活AP1转录因子的整合子FT和FD同源的Unigene11264_All、参与拟南芥光周期调控基因CO (CONSTANS)的同源基因Unigene4344_All、在芥菜中促进抽薹整合因子SOC1的表达的AGL24或直接作用于抽薹决定基因LFY的同源基因Unigene46836_All、Unigene7820_All、CL10006.Contig2_All。因此,上述候选基因很有可能参与调控当归抽薹相关分子机制。本研究筛选到与当归抽薹相关的候选基因,有助于了解当归抽薹相关分子机制,为挖掘控制当归抽薹相关基因提供理论依据。
Angelica early bolting seriously affects the yield of Angelica,and the molecular genetic mechanism bolting is still unclear.This study used RNA-seq technology to sequence the leaves,stem segments,seeds,and roots of Angelica sinensis,and annotated all the assembled Unigenes to find the main candidate genes for regulating Angelica sinensis bolting,and performed functional analysis of candidate genes.The results showed that 7.1,7.63,7.23,and 6.59 Gb of data were obtained in the transcription of leaves,roots,seeds,and stems of Angelica,respectively.Protein prediction through protein databases such as Nr,Nt,Swisstorm,KEGG,KOG,Interpro,GO,Intersection,Overall,etc..71024,59931,49694,51832,55320,46908,35598,17133,79161 proteins were predicted,respectively.80.13%of the predicted proteins in all transcripts are homologous to carrots.A total of 2666 genes expressed differently in the KEGG pathway are involved in plant signaling;A total of 56 candidate genes were screened and 56 candidate genes were compared with the genes involved in the regulation of flowering in Arabidopsis,carrots,and mustard,and 56 candidate genes were compared with nucleic acids in NCBI.Unigene45683_All,Unigene11264_All,which is homologous to the direct control of bolting AP1 transcription factor,the integron FT which activates AP1 transcription factor and the FD homologous,and the homologous gene Unigene4344_All,that participates in the optical cycle regulatory gene CO(CONSTANS)are obtained.Promoted in mustard the expression of AGL24 by bolting integration factor SOC1 can also directly affect the homologous genes of bolting gene LFY,Unigene468836_All,Unigene7820_All,and CL10006.Contig 2_All.Therefore,the above candidate genes are likely to participate in the regulation of Angelica pull-related molecular mechanisms.In this study,the candidate genes associated with bolting of Angelica sinensis were selected,which is helpful to understand the bolting related molecular mechanism of Angelica sinensis,and provide theoretical basis for excavating and control
作者
郭李智
宗渊
席杏媛
李建民
刘宝龙
Guo Lizhi;Zong Yuan;Xi Xingyuan;Li Jianmin;Liu Baolong(College of Life Science,Qinghai Normal University,Xining,810008;Key Laboratory of Crop Molecular Breeding,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,810001)
出处
《分子植物育种》
CAS
北大核心
2021年第2期448-458,共11页
Molecular Plant Breeding
基金
青海省植物基因与基因组学研究创新服务平台项目(2018-ZT-T08)资助。
