摘要
目的研究慢性乙型肝炎(CHB)病人外周血单个核细胞(PBMC)内微小RNA-155(Mir-155)、核苷酸结合寡聚化结构域样3(NLRP3)表达与乙型肝炎病毒DNA(HBV-DNA)载量的相关性。方法选取2017年6月至2019年9月沧州市传染病医院收治的经肝脏穿刺病理确诊的CHB病人118例为研究对象,根据荧光定量聚合酶链式反应(RT-qPCR)检测HBV-DNA载量分为低载量组(HBV-DNA<1×10^5拷贝/毫升)51例,中载量组(1×10^5拷贝/毫升≤HBV-DNA1×10^7拷贝/毫升)38例,高载量组(HBV-DNA>1×10^7拷贝/毫升)29例,另选取同期门诊健康体检者118例为健康对照组。采用RT-qPCR检测两组PBMC内Mir-155、NLRP3 mRNA及蛋白表达水平,采用酶联免疫吸附试验(ELISA)检测血清白细胞介素-1β(IL-1β)、白细胞介素-18(IL-18)水平,采用Pearson法分析CHB病人Mir-155与NLRP3蛋白水平及二者分别与IL-1β、IL-18水平的相关性。结果与健康对照组比较,CHB病人血清肝功能指标天门冬氨酸氨基转移酶(AST)[(18.17±3.65)mmol/L比(52.06±10.57)mmol/L]、丙氨酸氨基转移酶(ALT)[(22.84±4.56)mmol/L比(58.93±11.74)mmol/L]、PBMC中Mir-155[(1.01±0.16)比(3.42±0.53)]、NLRP3 mRNA[(1.02±0.15)比(4.26±0.75)]水平均显著升高(P<0.05)。与HBV-DNA低载量组比较,中、高载量组CHB病人PBMC中Mir-155、NLRP3 mRNA及蛋白水平、血清IL-1β、IL-18水平依次增加(P<0.05)。随肝炎炎症分级增加、肝纤维化分期增加,PBMC中Mir-155、NLRP3 mRNA水平依次增加(均P<0.05)。CHB病人PBMC中Mir-155与NLRP3、IL-1β、IL-18水平均呈显著正相关(r=0.714、0.733、0.852,P<0.05),NLRP3水平与血清IL-1β、IL-18水平均呈显著正相关(r 0.685、0.812,P<0.05)。结论随HBV-DNA载量增加,CHB病人Mir-155、NLRP3水平增加,可能协同促进炎症反应影响CHB发生发展。
Objective To study the correlations between the expressions of microRNA-155(Mir-155),nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)in peripheral blood mononuclear cells(PBMC)and the load of hepatitis B virus gene(HBV-DNA)of patients with chronic hepatitis B(CHB).MethodsOne hundred and eighteen patients with CHB who were diagnosed by liver biopsy from June 2017 to September 2019 were selected as the study subjects.According to the detection of HBV-DNA load by fluorescence quantitative PCR(RT-qPCR),51 cases were assigned into low-load group(HBV-DNA<1×10^5 copies/mL),38 cases in medium-load group(1×10^5 copies/mL<HBV-DNA<1×10^7 copies/mL),29 cases in high-load group(HBV-DNA>1×10^7 copies/mL),and another 118 healthy outpatients in the same period were selected as control group.The levels of Mir-155 and NLRP3 in PBMC were detected by RT-qPCR,the levels of IL-1βand IL-18 in serum were detected by ELISA,and the levels of Mir-155 and NLRP3 in CHB patients were analyzed by Pearson method.ResultsCompared with the control group,the serum levels of AST[(18.17±3.65)mmol/L vs.(52.06±10.57)mmol/L],ALT[(22.84±4.56)mmol/L vs.(58.93±11.74)mmol/L]and the levels Mir-155[(1.01±0.16)vs.(3.42±0.53)],NLRP3 mRNA[(1.02±0.15)vs.(4.26±0.75)]in PBMC in CHB patients were significantly higher(P<0.05).Compared with HBV-DNA low-load group,the levels of Mir-155,NLRP3 mRNAs and proteins,IL-1βand IL-18 in PBMC of CHB patients in medium and highload groups increased in turn(P<0.05).The mRNA levels of Mir-155 and NLRP3 in PBMC increased successively with the increase of hepatitis inflammation grade and liver fibrosis stage(P<0.05).Mir-155 in PBMC of CHB patients was positively correlated with NLRP3,IL-1βand IL-18 levels(r=0.714,0.733,0.852,P<0.05),and the level of NLRP3 was positively correlated with the levels of serum IL-1βand IL-18(r=0.685,0.812,P<0.05).ConclusionWith the increase of HBV-DNA load,the levels of Mir-155 and NLRP3 in CHB patients increase,which may synergistically promote inflammation and aff
作者
刘宏伟
王玉华
LIU Hongwei;WANG Yuhua(Department of Liver Diseases,Cangzhou Infectious Diseases Hospital,Cangzhou,Hebei 061001,China;Department of Laboratory,Cangzhou People's Hospital,Cangzhou,Hebei 061001,China)
出处
《安徽医药》
CAS
2021年第2期373-377,共5页
Anhui Medical and Pharmaceutical Journal
