摘要
“精神益生菌”是一类对神经功能和行为具有调节效果的益生菌。近年来,随着“肠-脑轴”理论的成熟,“精神益生菌”的开发也成为防治神经和精神类疾病的热点研究之一。该研究采用慢性不可预知温和刺激(chronic unpredictable midstress,CUMS)建立抑郁小鼠模型,并对小鼠进行为期4周的复合乳酸菌(短双歧杆菌CCFM1025、婴儿双歧杆菌CCFM687、乳酸片球菌CCFM6432)干预治疗。结果表明,复合乳酸菌能够提高慢性应激小鼠的糖水偏好性,减少其在强迫游泳实验中的不动时间,并增加其在高架十字迷宫中开放臂的活动时间;其作用机制与减少血清中皮质酮和大脑前额叶皮质的促炎因子(TNF-α和IL-1β)水平相关。慢性应激小鼠还表现出显著的便秘症状,而复合乳酸菌能够通过提高肠道短链脂肪酸、5-羟色胺(5-hydroxytryptomine,5-HT)及其受体基因表达(Htr4)的水平,增加小鼠的排便速率。此外,益生菌干预小鼠的肠道色氨酸(L-tryptophan,L-Trp)与脑中色氨酸和5-HT的含量具有显著正相关性,这表明对肠道和大脑L-Trp/5-HT合成的调控,可能是益生菌缓解便秘和抑郁的共同机制。研究结果证实了该复合乳酸菌制剂作为精神益生菌的潜力,拓展了益生菌及其发酵制品的营养功能和应用范围,也为抑郁症及其伴随胃肠道功能紊乱的治疗提供了新的思路。
“Psychobiotics”are a class of probiotics that have regulatory effects on neurobehavioral functions.With the maturity of the“microbiota-gut-brain axis”theory,psychobiotics have been widely used in the prevention and treatment of psychiatric diseases.In this study,mice under the chronic unpredictable mild stimulus(CUMS)were treated with multiple probiotics(Bifidobacterium breve CCFM1025,Bifidobacterium infantis CCFM687,and Pediococcus lactis CCFM6432)for four weeks.Multiple probiotics intervention improved the sucrose preference,reduced the immobile time in forced swim test,and increased the activity in open arms of elevated plus maze of the chronically stressed mice.The mechanism of action correlated with the down-regulation of serum corticosterone and pro-inflammatory cytokines(TNF-α and IL-1β)in the prefrontal cortex.Chronically stressed mice also showed significant constipation symptoms.Probiotics treatment increased the defecation speed which was possibly through the elevation of cecum short-chain fatty acids,5-hydroxytryptamine(5-HT)and Htr4 gene expression.Besides,significantly positive correlations were observed between the intestinal L-tryptophan(L-Trp)and brain L-Trp/5-HT levels in the mice treated with probiotics,which indicates that the regulation of intestinal and brain L-Trp/5-HT synthesis may contribute to both constipation and depression.This study confirms the psychobiotic potential of the multiple probiotics,expands the application of probiotics,and provides novel insight for the treatment of depression and accompanied constipation.
作者
邹仁英
朱慧越
许梦舒
田培郡
张灏
赵建新
陈卫
王刚
ZOU Renying;ZHU Huiyue;XU Mengshu;TIAN Peijun;ZHANG Hao;ZHAO Jianxin;CHEN Wei;WANG Gang(School of Food Science and Technology,Jiangnan University Wuxi,214122,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2021年第3期1-9,共9页
Food and Fermentation Industries
基金
国家自然科学基金面上工程项目(31972052)。
关键词
精神益生菌
抑郁
便秘
肠-脑轴
短链脂肪酸
psychobiotics
depression
constipation
gut-brain axis
short-chain fatty acids
