摘要
文章系统研究了不同提取工艺对沙棘蛋白组成及α-淀粉酶抑制活性的影响。分别采用水提、盐提、碱溶酸沉、先醇提后碱溶酸沉、先碱溶酸沉后醇提这5种工艺提取沙棘蛋白,比较了沙棘蛋白的蛋白质含量、蛋白提取率、色泽、α-淀粉酶抑制活性、氨基酸组成和重金属含量,确定了提取沙棘蛋白的最优工艺。研究结果表明,最佳提取工艺为先醇提后碱溶酸沉,该工艺提取得到的沙棘蛋白的蛋白质含量为(77.86±2.34)%,蛋白质提取率为(73.18±1.8)%,氨基酸组成完全,且当浓度为1mg/mL时,α-淀粉酶抑制活性可以达到85%,80℃热处理不会影响其抑制活性;重金属含量检测表明,该蛋白中重金属含量均低于国家安全标准,可以用于食品工业生产。该研究可为沙棘蛋白的深加工与利用提供理论基础。
The effects of different extraction process on the protein composition andα-amylase inhibitory activity of sea buckthorn are systematically studied.There are five kinds of process for extracting sea buckthorn protein by using water,salt,alkali soluble acid,alcohol extraction combined with alkali soluble acid and alkali soluble acid combined with alcohol extraction respectively.The protein content,protein extraction rate,color,α-amylase inhibitory activity,amino acid composition and heavy metal content of sea buckthorn protein are compared and the optimal extraction process of sea buckthorn protein is determined.The results show that alcohol extraction combined with alkali soluble acid is the best process,and sea buckthorn protein content is(77.86±2.34)%,protein extraction rate is(73.18±1.8)%,amino acid composition is completely,α-amylase inhibitory activity can reach 85%for sea buckthorn protein at 1 mg/mL,and heat treatment at 80℃does not affect the inhibitory activity.The detection results of heavy metal content show that the heavy metal content in the protein is lower than the national safety standard and could be used in food industry.This study has provided a theoretical basis for further processing and utilization of sea buckthorn protein.
作者
李家炜
喻勤
叶健文
相欢
崔春
LI Jia-wei;YU Qin;YE Jian-wen;XIANG Huan;CUI Chun(Perfect(Guangdong)Daily Necessities Co.,Ltd.,Zhongshan 528400,China;School of Food Science and Engineering,South China University of Technology,Guangzhou 510640,China)
出处
《中国调味品》
CAS
北大核心
2021年第2期75-78,共4页
China Condiment
基金
广东省科技计划(2017ZD093)。
关键词
沙棘蛋白
不同工艺
氨基酸组成
α-淀粉酶抑制活性
重金属含量
sea buckthorn protein
different extraction process
amino acid composition
α-amylase inhibitory activity
heavy metal content
