摘要
目的:观察雷公藤红素对人急性早幼粒白血病(APL)荷瘤模型小鼠黏附分子及细胞生物学特性的影响。方法:将18只SCID beige小鼠尾静脉注射NB4细胞株(5×10^6/只)以构建人APL荷瘤模型,然后随机分为荷瘤组、三氧化二砷组和雷公藤红素组,另取6只不造模并设为对照组;3周后向对照组和荷瘤组腹腔注射生理盐水并进行对照、三氧化二砷按100μg/kg腹腔注射,雷公藤红素按3 mg/kg腹腔注射,4组均注射治疗3周。观察各组治疗后小鼠静脉血NB4细胞生物学特性及黏附分子的蛋白表达。结果:与对照组比较,荷瘤组血象检测显示中性粒细胞降低,早幼粒细胞、NB4细胞、B淋巴细胞和白细胞明显升高(P<0.05),血清P-选择素(P-selectin)、可溶性血管黏附分子-1(sVCAM-1)和可溶性细胞间黏附分子-1(sICAM-1)均明显升高(P<0.05),细胞周期显示,G0、G1期比例明显升高,S和G2-M期明显降低(P<0.05)。与荷瘤组比较,雷公藤红素组中性粒细胞升高,早幼粒细胞、NB4细胞、B淋巴细胞、白细胞、P-selectin、sVCAM-1和sICAM-1均明显降低(P<0.05),且雷公藤红素组G0、G1期比例降低,G2-M和S期增多(P<0.05)。其中,雷公藤红素组白细胞、NB4细胞、P-selectin及sVCAM-1和sICAM-1与三氧化二砷组比较差异有统计学意义(P<0.05)。结论:雷公藤红素可能除具有抑制APL荷瘤模型小鼠sVCAM-1和sICAM-1蛋白表达而降低肿瘤细胞的黏附性外,还可将肿瘤细胞阻滞于G2-M和S期,从而发挥对APL的治疗效应。
Objective:To observe the effects of tripterine on adhesion molecules and cell biological characteristics in mice with acute promyelocytic leukemia(APL) tumor.Methods:Eighteen SCID beige mice were caudal vein injected with NB4 cell lines(5 × 106/only) to construct a human APL tumor-bearing model,then the mice were divided into tumor-bearing model group,arsenic trioxide group and tripterine group randomly,and another 6 mice which didn’t construct model were set up as control group;after 3 weeks,the control group and the tumor-bearing model were intraperitoneally injected with normal saline as compared,arsenic trioxide was intraperitoneally injected according to 100 μ g/kg,and tripterine was intraperitoneally injected according to 3 mg/kg.Four groups were all injected for 3 weeks.The biological characteristics of NB4 cells and the expression of adhesion molecules in venous blood of mice after treatment were observed.Results:The neutrophil decrased and promyelocytes,NB4 cells,B lymphocytes and white blood cells increased in tumor-bearing group as compared with control group(P<0.05),and the expressions of serum P-selectin(P-selectin),soluble vascular adhesion molecule-1(soluble vascular adhesion molecule-1,sVCAM-1) and soluble intercellular adhesion molecule-1(soluble intercellular adhesion molecule-1,sICAM-1) all increased(P<0.05).The cell cycle showed that the proportion of G0 and G1 increased,but S,G2-M phases obviously decreased(P<0.05).Compared with the tumor-bearing group,the neutrophils increased in the tripterine group,and the expression of early promyelocyte,NB4 cell,B lymphocyte,leukocyte,P-selectin,sVCAM-1 and sICAM-1 protein decreased(P<0.05),moreover,the ratio of G0 and G1 decreased,the G2-M and S phases increased(P<0.05).Among which sVCAM-1 and sICAM-1 of leucocyte,NB4 cell,P-selectin and adhesion molecules in tripterine group showed significant difference as compared with arsenic trioxide group(P<0.05).Conclusion:Tripterine may not only inhibit the expression of sVCAM-1 and sICAM-1 proteins in APL t
作者
宋登鹏
吴胜英
SONG Deng-Peng;WU Sheng-Ying(Department of Pharmacy,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,Hubei Province,China;Basic Medical College,Hubei University of Medicine,Shiyan 442000,Hubei Province,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2021年第1期72-76,共5页
Journal of Experimental Hematology
基金
十堰市太和医院院级项目(2017JJXM056)。
