摘要
聚合物胶束是一种新型的给药系统,其疏水核适用于装载各种难溶性药物,作为疏水药物的载体可以增加疏水药物的水溶性和稳定性.采用直接溶解法将阿霉素(DOX)—脱氧胆酸钠(NaDC)复合物的混悬液作为药物加入到一定浓度的普朗尼克F127胶束分散体中,磁力搅拌5 h后用0.45μm的乙酸纤维素过滤器过滤,将滤液倒入10 mL容量瓶并用超纯水稀释至刻度,制备得负载阿霉素的胶束溶液,随后对负载阿霉素的胶束和F127胶束的理化性质进行考察.用芘荧光探针法测F127的临界胶束浓度(CMC);纳米粒度及Zeta电位分析仪测得不同温度下两种胶束的粒径和Zeta电位;紫外分光光度计(UV)测定载药胶束的载药率;透析法测定其体外释放情况.实验结果显示阿霉素的包封率为(3.03±0.24)%,载药率为(0.15±0.1)%;负载阿霉素胶束的粒径和电位在不同温度下存在差异,发现阿霉素用F127胶束溶液包裹后能改善其水溶性差、不稳定等性质,具有良好的应用前景.
Polymer micelles are a new type of drug delivery system, and its hydrophobic core is suitable for loading a variety of poorly soluble drugs. As a carrier of hydrophobic drugs, polymer micelles can increase the water solubility and stability of hydrophobic drugs. In this paper, doxorubicin(DOX)-sodium deoxycholate(NaDC) complex suspension was added as a drug to a certain concentration of Pluronic F127 micelle dispersion by direct dissolution method, and magnetically stirred for 5 h followed by 0.45 μm cellulose acetate filtration, and then the filtrate was poured into flask and diluted to 10 mL with ultrapure water to prepare a micelle solution loaded with doxorubicin. The critical micelle concentration(CMC) of F127 was measured by the europium fluorescence probe method;the particle size and Zeta potential of two micelles at different temperatures were measured by a nanometer particle size and Zeta potential analyzer;the drug-loaded glue was measured by ultraviolet spectrophotometer(UV). The drug load of the bundle and the release quantity in vitro was measured by dialysis. The experimental results showed that the encapsulation rate of doxorubicin was(3.03 ± 0.24) % and the drug loading was(0.15 ± 0.1) %, and that the particle size and potential of the doxorubicin-loaded micelles were different at different temperatures. After being coated with F127 micelle solution, the poor water solubility and instability of DOX were improved. The micelles have potential applications in the fields of clinical medicine and pharmaceutical nanotechnology.
作者
郭坤
史珂婷
李伟杰
GUO Kun;SHI Ke-ting;LI Wei-jie(School of Pharmacy,Southwest Minzu University,Chengdu 610041,China;School of Chemistry and Environment,Southwest Minzu University,Chengdu 610041,China)
出处
《西南民族大学学报（自然科学版）》
CAS
2021年第1期19-27,共9页
Journal of Southwest Minzu University(Natural Science Edition)
基金
西南民族大学2020年省级大学生创新创业训练计划项目(S202010656129)。
