摘要
目的:探讨亚砷酸As(Ⅲ)对人肺癌A549细胞氧化应激的诱导作用。方法:将处于对数生长期的A549细胞暴露于不同浓度的亚砷酸As(Ⅲ)溶液(以砷浓度计分别为0、2.5、5、10和20μg/mL)中培养24 h,采用CCK-8法检测细胞存活率;用2.5和5μg/mL的亚砷酸As(Ⅲ)溶液染毒A549细胞24 h,并设对照组(含10%胎牛血清的RPMI-1640培养基),采用ELISA法检测细胞培养液中的超氧化物歧化酶(SOD)浓度,荧光探针DCFH-DA检测细胞内活性氧(ROS)的表达水平。结果:随着亚砷酸As(Ⅲ)溶液染毒浓度的升高,A549细胞的存活率呈逐渐降低的趋势(r=0.99,P<0.05)。2.5和5μg/mL亚砷酸As(Ⅲ)溶液染毒24 h后,细胞培养液中SOD的浓度分别为(0.72±0.05)、(1.10±0.16)ng/mL,均高于对照组的(0.56±0.03)ng/mL(P<0.05);细胞内ROS的相对荧光强度分别为(169.31±6.13)%和(242.60±2.35)%,均高于对照组的(100±2.80)%(P<0.05)。结论:A549细胞暴露于亚砷酸As(Ⅲ)溶液可致细胞存活率降低,细胞培养液中SOD和细胞内ROS浓度均升高;氧化应激可能是亚砷酸As(Ⅲ)致A549细胞毒性的机制之一。
OBJECTIVE:To investigate the induction of oxidative stress by arsenious acid solution As(Ⅲ) in human lung cancer A549 cells.METHODS:Cultured A549 cells were exposed to arsenious acid solution,As(Ⅲ),at doses of 0,2.5,5,10 and 200 μg/mL for 24 h.Cell viabilities were determined using the CCK-8 assay.For cells exposed to 0,2.5,5 μg/mL doses for 24 h,superoxide dismutase(superoxide dismutase,SOD) in cell culture medium was detected using ELISA,and intracellular reactive oxygen species(ROS) were detected using fluorescent probe DCFH-DA.RESULTS:As exposure concentration of As(Ⅲ)increased,A549 cell viabilities were gradually decreased(r=0.99,P<0.05).The level of SOD in the 2.5,and 5 μg/mL exposure groups were(0.72±0.05),and(1.10±0.16) ng/mL,respectively,which were higher than that of the control group [(0.56±0.03) ng/mL](P<0.05).Relative fluorescence intensities of ROS were(169.31±6.13)%,and(242.60±2.35)% for the same two groups which were higher than that of the control group(100±2.80)%.CONCLUSION:As(Ⅲ) exposure caused significantly reduced cell survival rates,increased concentrations of SOD and ROS in A549 cells.Oxidative stress may therefore be an important mechanism for the toxicity of As(Ⅲ).
作者
赵俊伟
华辰凤
尚平平
谢复炜
李翔
ZHAO Junwei;HUA Chenfeng;SHANG Pingping;XIE Fuwei;LI Xiang(Key Laboratory of Tobacco Chemistry,Zhengzhou Tobacco Research Institute of China National Tobacco Corporation,Zhengzhou 450001,Henan,China)
出处
《癌变．畸变．突变》
CAS
2021年第1期28-31,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
郑州烟草研究院院长科技发展基金(322014CA0350)。
关键词
亚砷酸
氧化应激
人肺癌A549细胞
超氧化物歧化酶
活性氧
arsenious acid solution
oxidative stress
human lung cancer A549 cells
superoxide dismutase
reactive oxygen species
