摘要
【目的】建立鹿角蕨孢子萌发与快速繁殖技术体系,为鹿角蕨规模化育苗提供技术支撑。【方法】以鹿角蕨孢子为外植体,采用3种不同消毒方法进行孢子灭菌与萌发;利用正交设计法,探讨基本培养基(MS、改良1号和改良2号)及植物生长调节剂(6-BA、KT、NAA和IBA)等对绿色球状体(GGB)增殖、丛生芽增殖及生根培养等关键环节的影响,筛选出适宜的孢子消毒方法及GGB增殖、丛生芽增殖和生根培养基配方。【结果】适宜孢子消毒的方法为超声波清洗器振荡清洗40 min,污染率低,仅为6.7%,孢子萌发率高达100.0%;各试验因素对GGB增殖影响的主次关系为基本培养基>6-BA>CH>NAA,以改良1号为基本培养基较好,6-BA适宜浓度为0.5 mg/L,NAA适宜浓度为0.1 mg/L,CH适宜浓度为0.2 g/L,42 d平均增殖系数达5.5;各试验因素对丛生芽增殖影响的主次关系为IBA>6-BA>NAA>KT,IBA适宜浓度为0.5 mg/L,6-BA适宜浓度为0.3 mg/L,KT适宜浓度为0.1 mg/L,NAA适宜浓度为0.3 mg/L,60 d平均增殖系数达5.3;添加NAA 0.3 mg/L为最适浓度,生根率为100.0%;试管苗移栽60 d成活率达98.5%。【结论】超声波清洗器振荡清洗40 min能有效降低鹿角蕨孢子污染率,提高孢子萌发率。鹿角蕨GGB增殖培养基以改良1号+6-BA 0.5mg/L+NAA 0.1 mg/L+CH 0.2 g/L较适宜,丛生芽增殖培养基以改良2号+6-BA 0.3 mg/L+KT 0.1 mg/L+NAA 0.3 mg/L+IBA 0.5 mg/L较适宜,生根培养基以改良1号+NAA 0.3 mg/L较适宜。
【Objective】The technique system of spore germination and rapid propagation of Platycerium wallichii Hook.was established to provide technical support for large-scale breeding of P.wallichii.【Method】The spores of P.wallichii were explants,they were sterilized and germinated by three different disinfection methods.The orthogonal design was used to study the effects of the key factors such as culture medium(MS,Improvement#1 and Improvement#2),phytohormone(6-BA,KT,NAA and IBA)on the proliferation of green globular body(GGB),multiplication of cluster buds and rooting culture,the suitable spore disinfection method and the medium formula of GGB multiplication,cluster bud multiplication and rooting were selected.【Result】The suitable method of spore disinfection was washed with ultrasonic wave cleaner for 40 min,the contamination rate was only 6.7%,the spore germination rate was as high as 100.0%.The primary and secondary relationship of each experimental factor to the bud multiplication was the basic medium>6-BA>CH>NAA.The best multiplication medium was Improvement#1,the proper concentrations were 6-BA 0.5 mg/L,NAA 0.1 mg/L,and CH 0.2 g/L,resulted in an averaged propagation coefficient of 5.5 in 42 d.The primary and secondary relationship of each experimental factor to the multiplication of GGB was IBA>6-BA>NAA>KT,the optimum concentration of IBA was 0.5 mg/L,6-BA was 0.3 mg/L,KT was 0.1 mg/L,the optimum concentration of NAA was 0.3 mg/L,the average multiplication coefficient was 5.3 on 60 d.The optimum concentration of NAA was 0.3 mg/L,the rooting rate was 100.0%,and the survival rate of plantlets transplanted in vitro was 98.5%on 60 d.【Conclusion】When spores are washed with ultrasonic wave cleaner for 40 min,the disinfection effect is good and the germination rate is high,the suitable medium for GGB proliferation is Improvement#1+6-BA 0.5 mg/L+NAA 0.1 mg/L+CH 0.2 g/L,the best medium for multiplication of cluster buds is Improvement#2+6-BA 0.3 mg/L+KT 0.1 mg/L+NAA 0.3 mg/L+IBA 0.5 mg/L,and the best mediu
作者
叶秀仙
陈艺荃
方能炎
吴建设
钟淮钦
YE Xiu-xian;CHEN Yi-quan;FANG Neng-yan;WU Jian-she;ZHONG Huai-qin(Institute of Crop Sciences,Fujian Academy of Agricultural Science,Fuzhou 350013,China;Institute of Agricultural Engineering and Technology,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2020年第11期2773-2780,共8页
Journal of Southern Agriculture
基金
福建省属公益科研院所基本科研专项(2017R1026-9)
福建省农业科学院特色花卉创新团队项目(STIT2017-2-9)。
关键词
鹿角蕨
超声波
绿色球状体
正交设计
快速繁殖
Platycerium wallichii Hook.
ultrasonic wave
green globular body
orthogonal design
rapid propagation
