摘要
目的探究丙泊酚对人神经胶质母细胞瘤(U343)活性、凋亡、侵袭和转移能力的影响。方法将处于对数生长期的人神经胶质母细胞瘤(U343)随机分为4组,分别为N组、S2组(Propofol 2μmol/L)、S5组(Propofol 5μmol/L)及S10组(Propofol10μmol/L)。除N组外,其余实验组加入相应浓度的丙泊酚,四组均在常氧培养箱培养48 h,采用CCK-8法检测神经胶质母细胞瘤(U343)活力,流式法检测细胞的凋亡,划痕实验检测细胞迁移能力,蛋白免疫印迹法检测凋亡相关蛋白Caspase-3﹑细胞因子VEGF的表达量。结果 CCK-8检测显示,S2组、S5组及S10组OD值低于N组,差异有统计学意义(P<0.05),且随着丙泊酚浓度的升高细胞活力降低,OD值降低。流式检测显示,S2组、S5组及S10组细胞凋亡率和死亡率高于N组,差异有统计学意义(P<0.05),且随着培养皿中丙泊酚浓度的升高,细胞凋亡(早期凋亡+晚期凋亡)率和死亡率升高;划痕实验显示:S2组、S5组及S10组迁移率低于N组,差异有统计学意义(P<0.05),且随着培养皿丙泊酚浓度升高,迁移能力降低;蛋白免疫印迹法显示,S2组、S5组及S10组凋亡相关蛋白Caspase-3表达高于N组,VEGF蛋白表达低于N组,差异有统计学意义(P<0.05)。结论丙泊酚能降低人神经胶质母细胞瘤(U343)的活力,增加细胞凋亡,抑制肿瘤细胞的增殖和迁移,且抑制程度10μmol/L>5μmol/L>2μmol/L。
Objective To explore the effects of propofol on the activity,apoptosis,invasion and metastasis of human glioblastoma(U343).Methods Human glioblastoma(U343)in the logarithmic growth phase was randomly divided into 4 groups,namely group N,group S2(Propofol 2μmol/L),and group S5(Propofol 5μmol/L)And S10 group(Propofol 10μmol/L).Except for group N,the other experimental groups were added with propofol of corresponding concentration.All four groups were cultured in a normoxic incubator for 48 h.The viability of glioblastoma(U343)was detected by the CCK-8 method,and the cells were detected by flow cytometry.Scratch test to detect cell migration ability,Western blotting method to detect the expression of apoptosis-related protein Caspase-3 and cytokine VEGF.Results The CCK-8 test showed that the OD values of the S2,S5 and S10 groups were lower than those of the N group,the difference was statistically significant(P<0.05).and as the concentration of propofol increases,cell viability decreases and OD value decreases.Flow cytometry showed that the apoptotic rate and death rate of S2,S5 and S10 groups were higher than those of N group,the difference was statistically significant(P<0.05),and with the increase of the propofol concentration in the culture dish,the rate of apoptosis(early apoptosis+late apoptosis)and mortality increased;the scratch experiment showed that the migration rate of S2,S5 and S10 groups was lower than N group,the difference was statistically significant(P<0.05),and as the concentration of propofol in the culture dish increased,the migration ability decreased.Western blotting showed that the expression of apoptosis-related protein Caspase-3 in groups S2,S5 and S10 was higher than that of group N,and the expression of VEGF protein was lower than group N,the difference was statistically significant(P<0.05).Conclusion Propofol can reduce the viability of human glioblastoma(U343),increase cell apoptosis,inhibit the proliferation and migration of tumor cells,and the degree of inhibition is 10μmol/L>5μm
作者
王昆锋
陈功
丁洁
WANG Kun-feng;CHEN Gong;DING Jie(Department of Anesthesiology,the Fifth Affiliated Hospital of Shenzhen University/Shenzhen Baoan Central Hospital,Shenzhen 518000,Guangdong,China)
出处
《医学信息》
2021年第2期86-89,共4页
Journal of Medical Information
