摘要
为探究砷(As)胁迫下典型湿地挺水植物香蒲(Typha angustifolia L.)的生长响应及其对砷的耐受性,采用室内模拟方式,通过外源添加Na2HAsO4·7H2O,设置不同As质量比的胁迫生境(w(As)分别为0、50、100、150、200、600 mg·kg^(−1)),探析香蒲生长、生理特性对砷的响应及砷的积累特征,综合评价香蒲对砷的耐受性.研究结果表明香蒲株高、根长、植株干重和侧芽数等生长指标对砷胁迫均表现出低促高抑的响应,香蒲体内叶绿素、丙二醛(MDA)和还原型谷胱甘肽(GSH)含量亦呈先增后降趋势,在土壤w(As)为100~150 mg·kg^(−1)处理条件下各指标达到峰值,总体而言香蒲地上部对砷胁迫较为敏感,地下部表现出较强的抗逆性;不同质量比砷胁迫下香蒲不同部位对砷的积累与转移存在差异,其积累能力大小为地下部>地上部、枯叶>新叶,转运系数较小,表明香蒲对砷的富集集中在地下部.应用隶属函数及主成分分析对不同w(As)胁迫下的香蒲综合评价表明,香蒲对砷具有较强的耐受性.在砷污染河湖滨湿地及土壤治理中,香蒲具有一定的潜在应用价值,研究结果为砷污染河湖的植物修复提供理论依据和技术支撑.
In order to investigate the growth response of typical wetland Typha angustifolia L.to arsenic(As)stress and its tolerance,plant growth and physiological property responding to As stress,As accumulation characteristics as well as As tolerance efficiency were examined in Typha angustifolia L.grown under different As stress levels(0,50,100,150,200,600 mg·kg^(−1)).The results showed that:①Plant height,root length,shoot and root dry weight,and lateral shoot number were increased by low-level As while decreased by high-level As.Chlorophyll,MDA and GSH contents showed a similar response and all reached the highest level under 100–150 mg·kg^(−1) As.Besides,the roots were less sensitive than the shoots,showing greater As resistance.②The As concentration and distribution in Typha were varied by different As stresses,the As accumulation capacity ranked as follow:shoots>roots and dead leaves>new leaves,indicating that As is predominantly concentrating in the underground part of Typha.③The membership function and principal component analysis under various As concentrations showed that Typha is highly resistant to As.The result indicated that Typha may be helpful in the remediation of As-contaminated river lakeside wetlands,providing theoretical basis and technical support for phytoremediation of As-polluted rivers or lakes.
作者
任伟
杨桂英
刘云根
刘雪
李梦莹
赵蓉
王妍
REN Wei;YANG Gui-ying;LIU Yun-gen;LIU Xue;LI Meng-ying;ZHAO Rong;WANG Yan(College of Ecology and Soil&Water Conservation,Southwest Forestry University,Kunming 650224,Yunnan,China;Yangtze Three Gorges Technology and Economy Development Co.,Ltd.,Beijing 100038,China;Research Center of Water Science and Engineering,Southwest Forestry University,Kunming 650224,Yunnan,China)
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第1期164-173,共10页
Journal of Yunnan University(Natural Sciences Edition)
基金
国家自然科学基金(21767027,41761098)
云南省应用基础研究计划(2018FB044)
云南省教育厅科学研究基金(2018Y121).
关键词
砷胁迫
香蒲
生长
积累
耐受性
arsenic stress
Typha angustifolia L.
growth
accumulation
tolerance
