摘要
该文立足科教结合背景,将癌胚抗原检测综合性实验应用到制药工程专业实验教学中,涵盖纳米技术、免疫分析技术、微流控芯片技术和仪器分析技术等多门前沿知识。实验基于表面增强拉曼免疫测定方法,制备了免疫金溶胶来提高拉曼检测灵敏度,制备了的Fe3O4@Au磁性纳米粒子用于富集信号,将金纳米粒子和磁性纳米粒子分别偶联癌胚抗原单克隆抗体后,在玻璃芯片中与测定抗原充分混合,形成夹心免疫复合物,最终通过精密仪器拉曼光谱进行定量分析。该实验与传统ELISA双抗体夹心法相比,具有检测时间短、操作简单和灵敏度高等优点。
Based on the background of combination of science and education, the comprehensive experiment of carcinoembryonic antigen determination is applied to the experimental teaching of pharmaceutical engineering specialty, covering nanotechnology, immunoassay technology, microfluidic chip technology and instrument analysis technology. In the experiment, immunogold sol is prepared by surface enhanced Raman immunoassay to improve the sensitivity of Raman detection. The Fe3O4@Au magnetic nanoparticles are prepared for signal enrichment. After coupling the gold nanoparticles and magnetic nanoparticles with the anti proto monoclonal antibody of carcinoembryon respectively, they are fully mixed with the detected antigen in the glass chip to form sandwich immune complex. Finally, the quantitative analysis is carried out by the precise instrument Raman spectrum. Compared with the traditional ELISA double antibody sandwich method, this experiment has the advantages of short detection time, simple operation and high sensitivity.
作者
高志刚
潘悦
罗勇
宋其玲
王世盛
汪晴
GAO Zhigang;PAN Yue;LUO Yong;SONG Qiling;WANG Shisheng;WANG Qing(National Experimental Teaching Demonstration Center for Chemical Engineering Comprehensive,Dalian University of Technology,Dalian 116024,China)
出处
《实验技术与管理》
CAS
北大核心
2020年第11期53-57,共5页
Experimental Technology and Management
基金
2018年辽宁省自然科学基金项目(ZX20180651)。
关键词
科教结合
制药工程
综合性实验
表面增强拉曼
combination of science and education
pharmaceutical engineering
comprehensive experiment
surface enhanced Raman
