摘要
目的揭示小鼠出生后早期耳蜗柯蒂氏器是否存在巨噬细胞及柯蒂氏器巨噬细胞形态和分布的变化。方法选1~4周龄的C57BL/6J小鼠,解剖取耳蜗基底膜。CD45抗体(一种全白细胞标记物)染色耳蜗基底膜,F4/80(巨噬细胞专有蛋白标记物)确认巨噬细胞,碘化丙锭标记细胞核,荧光显微镜下观察CD45染色阳性细胞的形态和分布变化。结果在出生后早期,小鼠耳蜗基底膜的中阶面柯蒂氏器存在巨噬细胞,巨噬细胞胞体及细胞核呈不规则形状。巨噬细胞定位于Hensen与Claudius细胞之间,分布于全耳蜗基底膜。自小鼠出生后第17天开始,一些巨噬细胞出现核消失,留下无核的细胞残余体。巨噬细胞的退变从耳蜗的底回向顶回发展。出生后1~2周小鼠耳蜗基底膜顶回、中回和底回的巨噬细胞长轴的平均直径均大于出生后3~4周小鼠(P=0.006,P=0.043,P=0.001,t检验)。结论出生后早期小鼠耳蜗柯蒂氏器存在巨噬细胞,随着感觉上皮发育成熟,这些细胞出现发育性死亡。推论柯蒂氏器巨噬细胞自耳蜗基底膜底回到顶回梯度的退化可能在耳蜗感觉上皮的发育中起到一定的作用。
Objective To determine whether macrophages are present in the organ of Corti,and their changes in morphology and distribution during early postnatal cochlear development in mice.Methods Cochleae were collected from C57BL/6J mice at ages of postnatal 1-4 weeks.The basilar membrane was immunolabeled with an antibody against CD45,a receptor tyrosine phosphatase presenting on all hematopoietic/bone marrow derived leukocytes.Macro‐phages were confirmed using F4/80,a macrophage-specific marker.Propidium iodide(PI)was used to label the nucleus.Morphology and distribution of CD45 and F4/80 staining positive cells were observed under a fluorescence microscopy.Results CD45 staining positive cellular structures were identified on the scala media side of the basilar membrane during early stages of postnatal development.Macrophages and their nuclei were irregular in shape.These macrophages were located beneath the cells of the organ of Corti,along the junction between Hensen's cells and Claudius'cells,and were visible in the apical,middle and basal cochlear turns.Starting at postnatal day 17,nuclei in some macrophages disappeared,leaving behind anucleated residual bodies.Macrophage degradation developed from basal to apical turns.The average length of macrophages at postnatal 1-2weeks was greater than that at postnatal 3-4weeks(P<0.05,t-test).Conclusion Macrophages are present in the organ of Corti at early stages of postnatal development.As the sensory epithelium matures,these cells undergo a development death.It is suggested that the degeneration of macrophages from the basal to apical turns may play a role in the development of cochlear sensory epithelium.
作者
杨卫平
许阳
胡博华
杨仕明
YANG Weiping;XU Yang;HU Bohua;YANG Shiming(College of Otolaryngology Head and Neck Surgery,Chinese PLA General Hospital,Chinese PLA Medical School,Beijing,100853 China;National Clinical Research Center for Otolaryngologic Diseases,Beijing,100853 China;State Key Lab of Hearing Science,Ministry of Education,Beijing,100853 China;Beijing Key Lab of Hearing Impairment Prevention and Treatment,Beijing,100853China;Department of Respiratory Diseases,Chinese PLA General Hospital,Beijing,100853 China;Center for Hearing and Deafness,State University of New York at Buffalo,Buffalo,NY 14214,USA)
出处
《中华耳科学杂志》
CSCD
北大核心
2021年第1期77-82,共6页
Chinese Journal of Otology
基金
国家自然基金资助项目(No:30973304)
美国NIH资助项目(NIDCD RO1DC010154)。
关键词
巨噬细胞
形态学
柯蒂氏器
耳蜗
发育
Macrophage
Morphology
Organ of Corti
Cochlea
Development
