摘要
该文旨在探讨生长抑制特异性蛋白1(growth arrest specific 1,Gas1)在早孕小鼠子宫内膜中的表达规律以及其在子宫内膜蜕膜化中的作用。通过免疫组化、Western blot和RT-qPCR检测Gas1在正常妊娠小鼠、假孕小鼠子宫内膜中的表达变化;分别构建体内小鼠子宫人工诱导蜕膜化模型和体外小鼠原代子宫内膜基质细胞人工诱导蜕膜化模型,通过Western blot和RT-qPCR检测Gas1在两种模型中的表达;在分离的小鼠原代子宫内膜基质细胞中过表达Gas1,通过Western blot、RT-qPCR和流式细胞术等方法检测其对蜕膜化、增殖和凋亡的影响;通过Western blot检测Gas1在人正常早孕蜕膜组织和自然流产蜕膜组织中的表达。结果表明,Gas1在小鼠孕D5、D6、D7的着床点表达显著低于着床旁;在体内和体外人工诱导蜕膜化模型中,Gas1在诱导组的表达明显低于非诱导组;而Gas1的上调则可通过影响细胞增殖和凋亡抑制蜕膜化。此外,Gas1在自然流产患者子宫内膜组织中的表达明显高于正常早孕组。该研究初步表明了Gas1可能通过介导细胞增殖和凋亡,影响小鼠子宫内膜基质细胞蜕膜化,从而在调控胚胎着床过程中起重要作用。
The purpose of this study was to explore the expression of Gas1 (growth arrest specific 1) in the endometrium of early pregnant mice and its role in decidualization.The expression of Gas1 in the endometrium of normal and pseudopregnant mice was detected by immunohistochemistry,Western blot and RT-qPCR.In vivo and in vitro models of artificial induced decidualization were constructed respectively,and the expression of Gas1 in the models was detected by Western blot and RT-qPCR.Gas1 was overexpressed in primary endometrial stromal cells,and its effects on decidualization,proliferation and apoptosis were detected by Western blot,RT-qPCR and flow cytometry.The results showed that Gas1 expression at implantation sites was significantly lower than that at the interimplantation sites of pregnant mice from D5 to D7.Artificial-induced decidualiazation inhibited the expression of Gas1 both in vivo and in vitro.Overexpression of Gas1 disturbed the proliferation and apoptosis of stromal cells and impaired decidualization.In addition,the expression of Gas1 in endometrial tissues of spontaneous abortion patients was significantly higher than that in the normal early pregnancy group.This study tentatively demonstrated that Gas1 might affect the decidualization of mouse endometrial stromal cells by mediating cell proliferation and apoptosis,thereby playing an important role in embryo implantation.
作者
谭淇蔓
徐翰婷
李南燕
刘学庆
何俊琳
丁裕斌
王应雄
高茹菲
陈雪梅
TAN Qiman;XU Hanting;LI Nanyan;LIU Xueqing;HE Junlin;DING Yubin;WANG Yingxiong;GAO Rufei;CHEN Xuemei(Joint International Research Laboratory of Reproductive and Development,Department of Reproductive Biology,School of Public Health and Management,Chongqing Medical University,Chongqing 400016,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2020年第12期2105-2115,共11页
Chinese Journal of Cell Biology
基金
国家自然科学基金(批准号:81573175)
重庆市自然科学基金项目(批准号:cstc2018jcyjAX0315)资助的课题。
关键词
胚胎植入
子宫内膜蜕膜化
凋亡
Gas1
embryo implantation
endometrial decidualization
apoptosis
Gas1
