摘要
【目的】筛选出竹叶兰各生长阶段适宜的培养基配方,建立竹叶兰组培快繁体系。【方法】以竹叶兰种子为材料,采用1/2 MS固体培养基,设计正交试验比较不同种类植物生长调节剂和添加物的浓度配比对竹叶兰种子无菌萌发、芽增殖生根等关键环节的影响。【结果】(1)竹叶兰种子萌发的最适培养基为1/2 MS+琼脂7 g·L^-1+蔗糖30.0 g·L^-1+活性炭1 g·L^-1+椰子乳100 mL·L^-1+NAA 1.0 mg·L^-1+6-BA 2.0 mg·L^-1+花宝1号0.5 g·L^-1,萌发率达83.79%;(2)芽增殖的最适培养基为1/2 MS+琼脂7 g·L^-1+蔗糖30 g·L^-1+活性炭1 g·L^-1+IBA 1 mg·L^-1+6-BA 3 mg·L^-1+NAA0.5 mg·L^-1+100 g·L^-1土豆泥,增殖率1.36;(3)芽生根培养的最适培养基为1/2 MS+琼脂7 g·L^-1+蔗糖30 g·L^-1+活性炭1 g·L^-1+IBA 0.2 mg·L^-1+6-BA 2 mg·L^-1+NAA(0.2~0.5)mg·L^-1+100 g·L^-1香蕉泥;(4)炼苗移栽的最适培养基质为珍珠岩∶泥炭土=1∶1,成活率高达98%,移栽效果良好。【结论】适宜的椰子乳有利于竹叶兰种子无菌萌发,通过正交试验所建立的竹叶兰组培快繁体系,可为竹叶兰野生种质资源的保护和开发利用提供保障。
【Objective】Formulation of culture media was optimized to facilitate the seed germination,tissue propagation,and various developmental stages of Arundina graminifolia.【Method】An orthogonal experiment was conducted to optimize the media for aseptic germination of the seeds and development of the seedlings of A.graminifolia using 1/2 MS as the base.Application of growth regulators and additives in the solid medium formulation was evaluated in accordance to the seed germination,seedling growth,root development and transplanting efficiency.【Result】The optimum media for various developmental stages of A.graminifolia were determined to include(1)1/2 MS+7 g agar·L^-1+30.0 g sucrose·L^-1+1 g activated carbon·L^-1+100 mL coconut milk·L^-1+1.0 mg NAA·L^-1+2.0 mg 6-BA·L^-1+0.5 g Huabao No.1·L^-1 to reach a seed germination rate of 83.79%.(2)1/2 MS+7 g agar·L^-1+30 g sucrose·L^-1+1 g activated carbon·L^-1+1 mg IBA·L^-1+3 mg 6-BA·L^-1+0.5 mg NAA·L^-1+100 g mashed potato·L^-1 to achieve an average bud proliferation rate of 1.36.(3)1/2 MS+7 g agar·L^-1+30 g sucrose·L^-1+1 g activated carbon·L^-1+0.2 mg IBA·L^-1+2 mg 6-BA·L^-1+0.2-0.5 mg NAA·L^-1+100 g banana puree·L^-1 for an optimal root development.(4)Perlite:peat soil mixture at 1∶1 ratio to yield a seedling survival rate of98%with desirable transplanting and acclimatization results.【Conclusion】Coconut milk in culture medium was found to significantly benefit the seed germination.The individually formulated media for various growth stages of A.graminifolia seedlings would help ensure the success in preserving the wild germplasms.
作者
夏春英
谢小敏
刘江枫
郑诚乐
XIA Chunying;XIE Xiaomin;LIU Jiangfeng;ZHENG Chengle(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Management Office of Yu Shan Scenic Area,Fuzhou,Fujian 350001,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2020年第11期1215-1221,共7页
Fujian Journal of Agricultural Sciences
基金
福州市园林局科研项目(201404)。
关键词
竹叶兰
无菌萌发
炼苗移栽
正交设计
组织培养
Arundina graminifolia(D.Don)Hochr
aseptic germination
acclimatization and transplant
orthogonal design
tissue culture
