摘要
目的:建立高效液相色谱法测定大鼠血浆中苍耳亭的浓度。方法:采用高效液相色谱法,色谱柱为Hypersil ODS2柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(V∶V=52∶48),流速为1.0 ml/min,检测波长为278 nm,柱温为25℃。血浆样品经二氯甲烷萃取富集,10μl进样检测。结果:苍耳亭质量浓度在0.1~10μg/ml(R^2=0.9997)范围内呈现良好的线性关系,定量下限为0.1μg/ml。苍耳亭低、中及高浓度的提取回收率分别为89.19%、83.56%及96.72%,日内和日间精密度分别≤5.47%和8.65%。结论:本方法灵敏快速简单,适用于体内苍耳亭的浓度分析,为药动学研究提供方法依据。
OBJECTIVE:To establish the high performance liquid chromatography(HPLC)method for determination of concentration of xanthine in rat plasma.METHODS:HPLC methods was adopted with Hypersil ODS2 column(250 mm×4.6 mm,5μm).The mobile phase was methanol-water(V∶V=52∶48),the flow rate was 1.0 ml/min,the detection wavelength was 278 nm,and the column temperature was 25℃.The plasma samples were extracted and enriched with dichloromethane,and 10μl injection was used for detection.RESULTS:The calibration curve of xanthatin in plasma showed good linear correlation in the range from 0.1 to 10μg/ml(R^2=0.9997)and the lower limit of concentration was 0.1μg/ml.The extraction recovery rates of low,medium and high concentration of Xanthatin was respectively 89.19%,83.56%and 96.72%,and the intra-day and inter-day precision was respectively≤5.47%and 8.65%.CONCLUSIONS:This method is sensitive,rapid and simple,and is suitable for the analysis of the concentration of xanthine in vivo,and provides basis for pharmacokinetic research.
作者
曹秀
韩静文
吴妍
李会婷
马旖旎
陈卫东
史天陆
CAO Xiu;HAN Jingwen;WU Yan;LI Huiting;MA Yini;CHEN Weidong;SHI Tianlu(School of Pharmacy,Anhui University of Chinese Medicine,Anhui Hefei 230012,China;Dept.of Pharmacy,the First Affiliated Hospital of University of Science and Technology of China,Anhui Provincial Hospital,Anhui Hefei 230036,China)
出处
《中国医院用药评价与分析》
2020年第12期1417-1420,共4页
Evaluation and Analysis of Drug-use in Hospitals of China
基金
安徽省自然科学基金面上项目(No.1708085MH225)。
关键词
苍耳亭
高效液相色谱法
血药浓度
大鼠
Xanthatin
High performance liquid chromatography
Plasma concentration
Rat
