摘要
通过薄层色谱法对化妆品中芦荟进行定性鉴别,并考察不同厂家、温度、相对湿度条件下对薄层色谱分离效果的影响,采用高效液相色谱法测定化妆品中芦荟苷含量。实验结果表明,供试品薄层色谱图斑点清晰、专属性强,与对照品、对照药材色谱图斑点均呈现良好的对应关系,可作为芦荟的定性鉴别依据;芦荟苷在0.2090~4.1800µg内与峰面积呈现良好线性关系,加样回收率为101.9%~102.7%,RSD为0.31%。该法简便、稳定、可靠,可作为化妆品中芦荟的质量控制方法。
The quality standard of Aloe vera in cosmetics was established.The Aloe vera in cosmetics was identified qualitatively by thin-layer chromatography(TLC).Meanwhile,the effects of different manufacturers,temperature and relative humidity on the separation effect of TLC were investigated.The content of Aloe glycoside was quantitatively analyzed by high performance liquid chromatography.The experimental results showed that,the TLC spots of the tested samples were clear and specific,and showed good correspondence with the spots of the reference substance and medicinal materials,which could be used as the basis for qualitative identification of Aloe vera.Aloe glycoside showed a good linearity with the peak area in the range of 0.2090~4.1800μg,and the recovery rate was 101.9%~102.7%with the RSD value of 0.31%.This method could be used as a quality control for Aloe vera in cosmetics because it was simple,stable and reliable.
作者
段鑫
方皓
吴雪梅
黄宇
胡昌江
DUAN Xin;FANG Hao;WU Xue-mei;HUANG Yu;HU Chang-jiang(Sichuan Green Herb Technology Development Co.,Ltd.,Chengdu,Sichuan 611193,China)
出处
《日用化学品科学》
CAS
2021年第1期26-28,33,共4页
Detergent & Cosmetics
基金
四川省科技厅重点研发项目(2018SZ0081)。
