摘要
经络是中医理论的核心概念.以往大量的研究表明,经络存在于间质中,其中可能有组织液的流动.荧光素钠是一种亲水的小分子荧光物质,用经络定位仪测量5只健康小型猪四肢的低电阻点(low impedance point, LIP)及其构成的循经低电阻线(low impedance line along meridians, LILM),然后在LIP及旁开高电阻点(high impedance point, HIP)注射微量荧光素钠.使用装有窄带滤色镜的数码相机与一组蓝光闪光灯采集注射前及注射后不同时刻的荧光迁移轨迹图像,观察荧光素迁移分布的特征.对部分体表荧光素轨迹,采用静脉显像仪定位浅表静脉后比较两者的位置.对部分体表荧光素轨迹,在干冰冷冻后观察其横切面的分布特征.结果发现,沿四肢的LILM共出现可重复的7条体表循经迁移轨迹,其中前肢外侧和内侧各有三条,后肢内侧有一条,迁移轨迹的平均长度为5.13 cm,而在HIP注射后仅出现接近圆形的局部扩散,两者的长度、宽度和长宽比均有极显著性差异(P<0.01).迁移轨迹与LILM的吻合度为81.4%,与体表静脉投影不重合,迁移轨迹在横切面的形状多为倒三角形,沿肌肉间隙分布,提示经络可能是一种未知的间质通道.
Meridians are a core concept in traditional Chinese medicine(TCM) theory. It has been shown in many previous studies that meridians exist in the interstitium, the space through which interstitial fluids flow. Sodium fluorescein(SF) is a small, fluorescent,hydrophilic molecule. SF was injected into the low impedance points(LIPs) or the high impedance points(HIPs) on the limbs of healthy mini-pigs, as determined by a low impedance meridian locator(WQ6 F30). Migration tracks were scanned by a digital camera(Canon 5 D2) with a narrow band filter and colored flash lamps prior to and after SF injection. The relationships between superficial veins and SF tracks were analyzed using a projection vein finder(V800 P). Tissues were frozen on dry ice, and the distribution of SF in the transverse sections of SF tracks was observed. Results reliably showed that seven migrating tracks along the low impedance line along meridians(LILM) were found when SF was injected at LIPs, three of which were on the outside of forelimb, three of which were on the inside of forelimb, and one of which was on the inside of hind limb. The average length of these migration tracks was5.13 cm. The migration tracks were 81.4% consistent with LILM, while no overlap was observed with veins. The shape of injected SF in the transverse sections was similar to an inverted triangle, and distributed in the muscle gap. No linear migration was observed when SF was injected at HIP, and the shapes of injected SF were nearly round by local diffusion. The length, width and length-width ratio of SF shapes between injections at LIPs and HIPs were significantly different(P<0.01). These results imply that the concept of the meridian in TCM may be an unknown interstitial channel.
作者
熊枫
宋晓晶
贾术永
王广军
李宏彦
顾鑫
王泽
张维波
XIONG Feng;SONG XiaoJing;JIA ShuYong;WANG GuangJun;LI HongYan;GU Xin;WANG Ze;ZHANG WeiBo(Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China;School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine,Beijing 100029,China;Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China)
出处
《中国科学：生命科学》
CSCD
北大核心
2020年第12期1453-1463,共11页
Scientia Sinica(Vitae)
基金
中央级公益性科研院所基本科研业务费专项资金(批准号:ZZ20191606)资助。
关键词
经络
荧光素钠
循经低电阻线
间质通道
小型猪
meridians
sodium fluorescein
low impedance line along meridians
interstitial channel
mini-pigs
