摘要
该实验通过确定经典名方清胃散水煎液的HPLC特征图谱,综合清胃散中指标性成分异阿魏酸、巴马汀、丹皮酚含量及出膏率,考察其煎煮工艺中饮片粉碎程度、煎煮水量、煎煮时长、滤材及煎煮容器等参数,对经典名方清胃散开发时煎煮工艺进行细化比较。通过建立清胃散的HPLC特征图谱方法,以特征图谱相似度、指标性成分浓度及出膏率为指标,对清胃散煎煮工艺各项参数进行细化优选。根据实验结果确定清胃散的煎煮工艺为:取处方量最粗粉饮片至陶瓷锅中,加水225 mL,武火煮沸后文火100 W煎煮50 min,用1层300目尼龙布过滤即得。不同煎煮方法清胃散特征图谱相似度均在0.9以上,确定煎煮工艺下清胃散中异阿魏酸、巴马汀以及丹皮酚质量浓度分别为40.74,26.73,65.73μg·mL^-1,出膏率为33.80%。该实验中确定的特征图谱能较好的表现清胃散全方信息及指标性成分,结合出膏率信息,能对经典名方清胃散的煎煮质量进行全方信息、指标性成分含量及出膏情况的综合评估,经细化考察后确定的煎煮工艺能更好反映清胃散煎煮的质量,便于控制和操作,为后续经典名方清胃散物质基准的开发研究提供基础,也能为其他经典名方煎煮工艺研究提供实验依据和参考。
In this experiment,by determination of the HPLC characteristic spectrum of the classical prescription Qingwei San decoction,the contents of isoferulic acid,palmatine and paeonol in Qingwei San decoction and the extraction rate were investigated.The factors such as the crushing degree of decoction pieces,the amount of decocting water,the decocting time,the filter material and the decocting container involved in Qingwei San decoction process were examined to make a detailed comparison of Qingwei San′s decoction processes during the development.HPLC characteristic spectrum method of Qingwei San was established,and then the decoction process parameters of Qingwei San were optimized,with the similarity of characteristic spectrum,the concentration of the index components and the extraction rate as indexes.The decoction process of Qingwei San was determined as follows:Qingwei San decoction pieces were weighed according to the prescription amount and pulverized into the most coarse powder;the powder was put in a ceramic pot,added with 225 mL water,heated to boiling,cooked for 50 minutes with gentle heat(100 W),and filtered with a layer of 300 mesh nylon cloth.The similarity of Qingwei San′s characteristics pectrum of different decoction methods was all above 0.9,and the concentration of isoferulic acid,palmatine and paeonol in Qingwei San under determined decoction process was 40.74,26.73,65.73μg·mL^-1,respectively,with an extraction rate of 33.80%.The characteristic spectrum determined in this experiment can better express the information and index components of Qingwei San,and if combined with the extraction rate information,it can provide the general information,index component content and extraction information.The decoction process after detailed investigation can better reflect the quality of Qingwei San decoction,with easier control and operation.It can provide a basis for the subsequent research and development of Qingwei San decoction standard,and can also provide experimental basis and reference for the d
作者
张琦
黄嘉怡
钟宛凌
刘蕾蕾
张钰明
杨琳洁
白洁
杜守颖
ZHANG Qi;HUANG Jia-yi;ZHONG Wan-ling;LIU Lei-lei;ZHANG Yu-ming;YANG Lin-jie;BAI Jie;DU Shou-ying(School of Chinese Materia Mediea,Beijing University of Chinese Medicine,Beijing 102488,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2020年第23期5607-5613,共7页
China Journal of Chinese Materia Medica
关键词
清胃散
经典名方
特征图谱
煎煮工艺
Qingwei San
classical prescription
characteristic spectrum
decoction process
