摘要
目的探讨丙型肝炎病毒核心蛋白结合蛋白6(hepatitis C virus core-binding protein 6,HCBP6)在脂肪性肝病患者中的表达及意义。方法收集2019年8月至2020年2月首都医科大学附属北京地坛医院确诊的30例脂肪性肝病患者为研究对象,其中酒精性脂肪性肝病(alcoholic liver disease,ALD)组10例,非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)组20例,另选取30例同期健康体检者为正常对照组。采用全自动生化分析仪检测血清生物化学指标,包括:丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸基转移酶(aspartate aminotransferase,AST)、总胆红素(total bilirubin,TBil)、直接胆红素(direct bilirubin,DBil)、总胆固醇(cholesterol,CHOL)和甘油三酯(triglyceride,TG)。采用Western blot和酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测血清HCBP6水平。结果脂肪性肝病组患者ALT [(80.16±21.26)U/L vs(29.20±3.58)U/L]、AST [(97.10±20.76)U/L vs(46.30±4.52)U/L]、CHOL [(4.41±0.09)mmol/L vs(3.77±0.08)mmol/L]和TG [(1.92±0.08)mmol/L vs(1.48±0.03)mmol/L]水平均显著高于正常对照组,差异有统计学意义(P均<0.05),TBil [(91.11±25.75)μmol/L vs(42.90±7.50)μmol/L]和DBil[(65.23±20.12)μmol/L vs(26.14±5.01)μmol/L]差异无统计学意义(P均> 0.05)。NAFLD组、ALD组及正常对照组ALT [(70.25±18.24)U/L vs(99.98±53.82)U/L vs(29.20±3.58)U/L]、AST[(91.35±26.09)U/L vs(98.60±35.93)U/L vs(46.30±4.52)U/L]、CHOL [(4.56±0.10)mmol/L vs(4.12±0.11)mmol/L vs(3.77±0.08)mmol/L]和TG [(1.97±0.02)mmol/L vs(1.81±0.09)mmol/L vs(1.48±0.03)mmol/L]水平差异有统计学意义(P均<0.05),TBil [(97.34±34.91)μmol/Lvs(78.67±35.21)μmol/L vs(42.90±7.50)μmol/L]和DBil [(69.34±27.35)μmol/L vs(57.01±27.24)μmol/L vs(26.14±5.01)μmol/L]差异无统计学意义(P均> 0.05)。其中,NAFLD组和ALD组ALT、AST、CHOL和TG水平均显著高于正常对照组(P均<0.05);NAFLD组CHOL水平显著高于ALD组(t=2.681,P=0.012),两组患者ALT�
Objective To investigate the expression and clinical significance of hepatitis C virus core-binding protein 6(HCBP6)in patients with fatty liver disease.Methods A total of 30 patients with fatty liver disease in Beijing Ditan Hospital,Capital Medical University from August 2019 to February 2020 were selected,including 10 cases in alcoholic fatty liver(ALD)group and 20 cases in non-alcoholic fatty liver disease(NAFLD)group.Another 30 healthy subjects were collected as normal control group.Automatic biochemical analyzer was used to detect the serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBil),direct bilirubin(DBil),cholesterol(CHOL)and triglyceride(TG).Western blot and enzyme linked immunosorbent assay(ELISA)were used to detect the serum level of HCBP6.Results ALT[(80.16±21.26)U/L vs(29.20±3.58)U/L],AST[(97.10±20.76)U/L vs(46.30±4.52)U/L],CHOL[(4.41±0.09)mmol/L vs(3.77±0.08)mmol/L]and TG[(1.92±0.08)mmol/L vs(1.48±0.03)mmol/L]levels of patients in fatty liver group were significantly higher than those of normal control group(all P<0.05),and there were no significant difference in TBil[(91.11±25.75)μmol/L vs(42.90±7.50)μmol/L]and DBil[(65.23±20.12)μmol/L vs(26.14±5.01)μmol/L](all P>0.05).There were significant differences in ALT[(70.25±18.24)U/L vs(99.98±53.82)U/L vs(29.20±3.58)U/L],AST[(91.35±26.09)U/L vs(98.60±35.93)U/L vs(46.30±4.52)U/L],CHOL[(4.56±0.10)mmol/L vs(4.12±0.11)mmol/L vs(3.77±0.08)mmol/L]and TG[(1.97±0.02)mmol/L vs(1.81±0.09)mmol/L vs(1.48±0.03)mmol/L]levels of patients in NAFLD group,ALD group and normal control group(all P<0.05),and there were no significant differences in TBil[(97.34±34.91)μmol/L vs(78.67±35.21)μmol/L vs(42.90±7.50)μmol/L]and DBil[(69.34±27.35)μmol/L vs(57.01±27.24)μmol/L vs(26.14±5.01)μmol/L](all P>0.05).ALT,AST,CHOL and TG levels of patients in NAFLD group and ALD group were significantly higher than those of normal control group(all P<0.05).The CHOL level of patients in NAFLD group was signif
作者
张雨
纪世博
李炜
庄立伟
李贲
全敏
段英
王笑梅
欧蔚妮
成军
邢卉春
Zhang Yu;Ji Shibo;Li Wei;Zhuang Liwei;Li Ben;Quan Min;Duan Ying;Wang Xiaomei;Ou Weini;Cheng Jun;Xing Huichun(Cental of Liver Diseases Division 3,Peking University Ditan Teaching Hospital,Beijing 100015,China;Central of Liver Diseases Division 3,Beijing Ditan Hospital,Capital Medical University,Beijing 100015,China)
出处
《中国肝脏病杂志（电子版）》
CAS
2020年第4期48-53,共6页
Chinese Journal of Liver Diseases：Electronic Version
基金
首都医科大学附属北京地坛医院青年人才发展基金“萌芽”支持计划(DTMY201801)
北京市医院管理中心扬帆计划(xmlx201837)
北京市医院管理中心消化内科学科协同发展中心项目(XXT26)
“十三五”艾滋病和病毒性肝炎等重大传染病防治(2018ZX10302206-003-006)
首都卫生发展科研专项(首发2020-1-2171)。
