摘要
目的:研究氯霉素泼尼松搽剂对小鼠急性特应性皮炎的治疗效果。方法:选取48只小鼠,分为空白组、模型组、对照组及实验低、中、高剂量组共6组,每组8只,除空白组外,其余5组小鼠均用二硝基氯苯丙酮溶液建立特应性皮炎模型,空白组、模型组给予生理盐水,对照组给予1%吡美莫司乳膏,实验组低、中、高剂量组分别给予相应浓度的氯霉素泼尼松搽剂,每天1次,7 d为1个疗程,疗程结束后24 h,检测小鼠血清IFN-γ、IL-4、IL-5、IgE水平、耳肿胀度、胸腺指数、脾脏指数、皮损厚度等指标,评价氯霉素泼尼松搽剂对小鼠特应性皮炎的治疗效果。结果:治疗7 d后实验组小鼠血清中IL-4、IL-5和Ig E水平低于模型组(P<0.05),IFN-γ水平高于模型组(P<0.05),耳肿胀度、胸腺指数、脾脏指数、皮损厚度中、高剂量组低于模型组(P<0.05)。结论:氯霉素泼尼松搽剂具有抗菌消炎、抗变态反应、调节免疫作用,可改善特应性皮炎症状,调节Th1/Th2细胞平衡,对小鼠特应性皮炎具有显著的治疗作用,临床上可用于类似炎性皮肤病的治疗。
Objective:To study the therapeutic effects of chloramphenicol prednisone liniment on acute atopic dermatitis in mice.Methods:Forty-eight mice were equally divided into the blank group,model group,control group and experimental groups(low dose group,medium dose group and high dose group),with 8 cases in each group.Except for the blank group,atopic dermatitis model was established by dinitrochlorophenone solution in all the other 5 groups.The blank group and model group were given normal saline.The control group was given 1%pimecrolimus cream.The experimental groups(low dose group,medium dose group and high dose group)were given corresponding concentration of chlorine respectively,once a day,7 d for 1 course of treatment.After treatment of 24 h,the therapeutic effects of chloramphenicol prednisone liniment on atopic dermatitis in mice were evaluated by indicators of serum IFN-γ,IL-4,IL-5,IgE,ear swelling,thymus indicator,spleen indicator and skin thickness.Results:After treatment of 7 d,the levels of IL-4,IL-5 and IgE in the experimental group were significantly lower than those in the model group(P<0.05),the levels of IFN-γwere significantly higher than those in the model group(P<0.05),the degree of ear swelling,thymus indicator,spleen indicator and skin thickness in the medium dose group and high dose group were significantly lower than those in the model group(P<0.05).Conclusion:Chloramphenicol prednisone liniment has anti-bacterial,anti-inflammatory,anti-allergic and immune-regulating effects,which can improve the symptoms of atopic dermatitis,regulate the balance of Th1/Th2 cells,and has significant therapeutic effects on atopic dermatitis in mice.Chloramphenicol prednisone liniment can be used for the treatment of similar inflammatory skin diseases.
作者
陈芳
袁昊
马朝晖
陈少秀
常明泉
Chen Fang;Yuan Hao;Ma Zhaohui;Chen Shaoxiu;Chang Mingquan(Taihe Hospital of Shiyan,Hubei Shiyan 442000,China;Hubei University of Medicine,Hubei Shiyan 442000,China)
出处
《儿科药学杂志》
CAS
2021年第1期4-7,共4页
Journal of Pediatric Pharmacy
基金
湖北省科技厅成果鉴定项目,编号EK2018A010355001436。
关键词
氯霉素泼尼松搽剂
特应性皮炎
疗效
chloramphenicol prednisone liniment
atopic dermatitis
efficacy