摘要
目的:探讨钙联蛋白A9(S100A9)和上皮间质转化相关蛋白波形蛋白(Vimentin)在子宫颈癌及其淋巴结中的表达及意义。方法:采用免疫组织化学方法检测99例子宫颈癌患者原发灶、淋巴结转移(LNM)灶及灶旁淋巴结和盆腔阴性淋巴结组织中S100A9和Vimentin的表达情况,根据术后病理检查结果分为LNM组(33例)和无淋巴结转移(NLNM)组(66例),分析S100A9和Vimentin表达与子宫颈癌LNM、转移前生态位的关系。结果:(1)S100A9和Vimentin在子宫颈癌原发灶和淋巴结转移灶主要表达于细胞质,在淋巴结组织主要表达于细胞膜;(2)S100A9和Vimentin在子宫颈癌原发灶中的阳性表达率分别为77.8%(77/99)、29.3%(29/99)。S100A9和Vimentin在子宫颈癌原发灶表达的阳性率均与LNM有关(P<0.05);(3)S100A9在LNM组子宫颈癌原发灶和转移灶表达的阳性率差异无统计学意义(P>0.05),但表达强度差异有统计学意义(P<0.05),而Vimentin阳性表达率和强度差异均无统计学意义(P>0.05);(4)S100A9在淋巴结中表达的阳性率:LNM组灶旁淋巴结(70.0%)>LNM组阴性淋巴结(37.3%)≈NLNM组阴性淋巴结(27.0%);Vimentin在淋巴结中表达的阳性率:LNM组灶旁淋巴结(75.0%)≈LNM组阴性淋巴结(72.3%)>NLNM组阴性淋巴结(43.7%);(5)S100A9和Vimentin在子宫颈癌原发灶和LNM灶中的表达强度具有显著的正相关性(r>0,P<0.05);(6)S100A9和Vimentin在子宫颈癌原发灶中的阳性表达率与转移淋巴结数目无明显关系(P>0.05),但S100A9(r=0.555,P<0.001)和Vimentin(r=0.227,P=0.02)表达强度随LNM数目增加而增加。结论:S100A9和Vimentin表达与子宫颈癌LNM相关,S100A9可能参与LNM、转移前生态位等过程,其机制可能涉及到上皮间质转化,S100A9和Vimentin有望作为评估子宫颈癌LNM的指标。
Objective:To explore the expression and significance of S100 A9 and epithelial-mesenchymal transition(EMT) related protein Vimentin in cervical cancer and its lymph nodes.Methods:Immunohistochemistry was used to detect the expression of S100 A9 and Vimentin in cervical cancer tissues,metastasized lymph nodes,its surrounding lymph node tissue and negative pelvic lymph nodes of 99 patients.The patients were divided into LNM group(33 cases) and non-lymph node metastasis(NLNM) group(66 cases) according to the postoperative pathological results.Relationship between the expression of S100 A9 as well as Vimentin and metastasis,premetastatic niche of lymph nodes were analyzed.Results:(1)S100 A9 and Vimentin were mainly expressed in cytoplasm in primary tumor issue and metastasized lymph nodes,and mainly in cell membranes in lymph node tissue.(2)The positive expression of S100 A9 and Vimentin in primary tumor tissues were 77.8%(77/99),29.3%(29/99)respectively.The positive expression had a significant correlation with lymph node metastasis(LNM)(P<0.05).(3)The positive rates of S100 A9 in primary tumors and metastasized lymph nodes were not significantly different(P>0.05),but the intensity was significantly different(P<0.05). The positive rate and intensity of Vimentin in primary tumors and metastasized lymph nodes were not significantly different(P>0.05).(4)S100 A9 positive rate in lymph nodes:lymph nodes adjacent to cancer in the LNM group(70.0%)>negative lymph nodes in the LNM group(37.3%) ≈negative lymph nodes in the NLNM group(27.0%).The positive rate of Vimentin in lymph nodes:lymph nodes adjacent to cancer in the LNM group(75.0%)≈negative lymph nodes in the LNM group(72.3%)>negative lymph nodes in the NLNM group(43.7%).(5)The intensity of S100 A9 and Vimentin in the primary tumors and metastasized lymph nodes showed a significant positive correlation(r>0,P<0.05).(6)The positive rate of S100 A9 or Vimentin in the primary tumors was not related to the number of lymph nodes(P>0.05),while the intensity increased with
作者
蒋艳平
张赛
王前
徐倩
张淑军
曹兰琴
JIANG Yanping;ZHANG Sai;WANG Qian(Gynecological Department of Xiangya Hospital,Central South University,Changsha Hunan 410008,China;Scientific Research Center of Xiangya Hospital,Central South University,Changsha Hunan 410008,China)
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2020年第12期920-925,共6页
Journal of Practical Obstetrics and Gynecology
基金
湖南省科技厅重点研发计划(编号:2020SK2073)。
